| Literature DB >> 22900320 |
Abstract
Cytochrome c552 was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be 14.1 kD.Entities:
Mesh：
Substances：
Year: 2003 PMID： 22900320
Source DB: PubMed Journal: Indian J Biochem Biophys ISSN： 0301-1208 Impact factor: 1.918