OBJECTIVES: The present study was conducted to ascertain whether cigarette smoke induces oxidative stress in the human pancreas concurrently with inflammation. MATERIALS AND METHODS: The influence of tobacco smoking on the serum level of interleukin-6 (IL-6), on the activities of glutathione peroxidase (GPx) and copper-zinc superoxide dismutase (Cu/Zn SOD) as well as on the metallothionein (MT) level in the pancreatic pseudocyst fluid and its immunohistochemical localization in tissues of non-smoking (n = 9) and smoking (n = 12) patients with diagnosed chronic pancreatitis (CP) was measured. The concentration of interleukin-6 and metallothionein was determined by means of ELISA and the radioisotopic method, respectively. The enzyme activities in the fluid were assayed by the colorimetric method. Samples of tissues of normal pancreas (n = 4) and CP (non-smoking n = 7; smoking n = 12) were verified histopathologically and then IL-6, MT and enzymes were localized by immunohistochemical staining using the monoclonal anti-human antibody. RESULTS: The concentrations of metallothionein and interleukin-6 were significantly higher in smoking patients with CP (as compared with the non-smoking population (p < 0.01; p < 0.001). Interestingly, the ratio of MT/IL-6 in smoking patients with CP was reduced in comparison to non-smoking patients (respectively: 1.1; 5.6). In smoking patients, a significant elevation of the Cu/Zn SOD and GPx activities was revealed as compared with the non-smokers (p < 0.04; p < 0.0007). These studies clearly demonstrate a moderate and strong expression of IL-6 and enzymes in acinar, islet and duct cells of smoking patients. CONCLUSIONS: These observations favor the role of the oxidative stress in the induction of pancreatitis associated with chronic cigarette smoke inhalation.
OBJECTIVES: The present study was conducted to ascertain whether cigarette smoke induces oxidative stress in the human pancreas concurrently with inflammation. MATERIALS AND METHODS: The influence of tobacco smoking on the serum level of interleukin-6 (IL-6), on the activities of glutathione peroxidase (GPx) and copper-zinc superoxide dismutase (Cu/Zn SOD) as well as on the metallothionein (MT) level in the pancreatic pseudocyst fluid and its immunohistochemical localization in tissues of non-smoking (n = 9) and smoking (n = 12) patients with diagnosed chronic pancreatitis (CP) was measured. The concentration of interleukin-6 and metallothionein was determined by means of ELISA and the radioisotopic method, respectively. The enzyme activities in the fluid were assayed by the colorimetric method. Samples of tissues of normal pancreas (n = 4) and CP (non-smoking n = 7; smoking n = 12) were verified histopathologically and then IL-6, MT and enzymes were localized by immunohistochemical staining using the monoclonal anti-human antibody. RESULTS: The concentrations of metallothionein and interleukin-6 were significantly higher in smoking patients with CP (as compared with the non-smoking population (p < 0.01; p < 0.001). Interestingly, the ratio of MT/IL-6 in smoking patients with CP was reduced in comparison to non-smoking patients (respectively: 1.1; 5.6). In smoking patients, a significant elevation of the Cu/Zn SOD and GPx activities was revealed as compared with the non-smokers (p < 0.04; p < 0.0007). These studies clearly demonstrate a moderate and strong expression of IL-6 and enzymes in acinar, islet and duct cells of smoking patients. CONCLUSIONS: These observations favor the role of the oxidative stress in the induction of pancreatitis associated with chronic cigarette smoke inhalation.
Authors: Tianxiao Huan; Roby Joehanes; Claudia Schurmann; Katharina Schramm; Luke C Pilling; Marjolein J Peters; Reedik Mägi; Dawn DeMeo; George T O'Connor; Luigi Ferrucci; Alexander Teumer; Georg Homuth; Reiner Biffar; Uwe Völker; Christian Herder; Melanie Waldenberger; Annette Peters; Sonja Zeilinger; Andres Metspalu; Albert Hofman; André G Uitterlinden; Dena G Hernandez; Andrew B Singleton; Stefania Bandinelli; Peter J Munson; Honghuang Lin; Emelia J Benjamin; Tõnu Esko; Hans J Grabe; Holger Prokisch; Joyce B J van Meurs; David Melzer; Daniel Levy Journal: Hum Mol Genet Date: 2016-11-01 Impact factor: 6.150
Authors: Thiruvengadam Muniraj; Dhiraj Yadav; Judah N Abberbock; Samer Alkaade; Stephen T Amann; Michelle A Anderson; Peter A Banks; Randall E Brand; Darwin Conwell; Gregory A Cote; Christopher E Forsmark; Timothy B Gardner; Andres Gelrud; Nalini Guda; Michele D Lewis; Joseph Romagnuolo; Bimaljit S Sandhu; Stuart Sherman; Vikesh K Singh; Adam Slivka; Gong Tang; David C Whitcomb; C Mel Wilcox Journal: Pancreatology Date: 2019-02-26 Impact factor: 3.996
Authors: Samuel Y Han; Darwin L Conwell; Philip T Diaz; Amy Ferketich; Christie Y Jeon; Dhiraj Yadav; Phil A Hart Journal: Pancreatology Date: 2022-08-11 Impact factor: 3.977