AIMS: To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains. METHODS AND RESULTS: Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween. CONCLUSIONS: Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay. SIGNIFICANCE AND IMPACTS OF THE STUDY: The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon.
AIMS: To assess the ability of vaginal lactobacilli to form biofilm under different culture conditions and to determine the relationship between their growth and the capability of biofilm formation by selected strains. METHODS AND RESULTS: Fifteen Lactobacillus strains from human vagina were tested for biofilm formation by crystal violet staining. Only Lactobacillus rhamnosus Centro de Referencia para Lactobacilos Culture Collection (CRL) 1332, Lact. reuteri CRL 1324 and Lact. delbrueckii CRL 1510 were able to grow and form biofilm in culture media without Tween 80. However, Lact. gasseri CRL 1263 (a non-biofilm-forming strain) did not grow in these media. Scanning electron microscopy showed that Lact. rhamnosus CRL 1332 and Lact. reuteri CRL 1324 formed a highly structured biofilm, but only Lact. reuteri CRL 1324 showed a high amount of extracellular material in medium without Tween. CONCLUSIONS: Biofilm formation was significantly influenced by the strain, culture medium, inoculum concentration, microbial growth and chemical nature of the support used for the assay. SIGNIFICANCE AND IMPACTS OF THE STUDY: The results allow the selection of biofilm-forming vaginal Lactobacillus strains and the conditions and factors that affect this phenomenon.
Authors: Abdelhamid A Hamdy; Nouran A Elattal; Magdy A Amin; Amal E Ali; Nahla M Mansour; Ghada E A Awad; Hassan M Awad; Mona A Esawy Journal: World J Microbiol Biotechnol Date: 2017-03-07 Impact factor: 3.312
Authors: Shweta Malik; Mariya I Petrova; Ingmar J J Claes; Tine L A Verhoeven; Pieter Busschaert; Mario Vaneechoutte; Bart Lievens; Ivo Lambrichts; Roland J Siezen; Jan Balzarini; Jos Vanderleyden; Sarah Lebeer Journal: Appl Environ Microbiol Date: 2013-05-24 Impact factor: 4.792
Authors: Monique S do Carmo; Francisca M F Noronha; Mariana O Arruda; Ênnio P da Silva Costa; Maria R Q Bomfim; Andrea S Monteiro; Thiago A F Ferro; Elizabeth S Fernandes; Jorge A Girón; Valério Monteiro-Neto Journal: Front Microbiol Date: 2016-10-27 Impact factor: 5.640