P Hu1, R Lai, D Xie. 1. Institute of Otology, Department of Otolaryngology and Head and Neck surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Abstract
OBJECTIVES/HYPOTHESIS: Aminoglycosides may decrease the expression of some proteins participating in ion-exchange in the cochlear lateral wall. Connexin 26 expression in the lateral wall may play a role in acquired hearing loss by maintaining the endocochlear potential and potassium concentration in the endolymph. We examined the effects of gentamicin on the expression of connexin 26 to obtain a better understanding of aminoglycoside ototoxicity. METHODS: We detected changes in connexin 26 protein and mRNA expression in the cochlear lateral wall using immunohistochemistry staining, western blotting, and real-time PCR in rats after administration of a single dose of gentamicin. RESULTS: The expression of connexin 26 increased over time in type III fibrocytes after gentamicin administration. Elevated protein levels were detected 3 h after the single injection of gentamicin; while, mRNA levels increased after 24 h. CONCLUSION: Connexin 26 plays an important role in the acute effects of high-dose gentamicin and is probably involved in the pathogenesis of ototoxic deafness.
OBJECTIVES/HYPOTHESIS: Aminoglycosides may decrease the expression of some proteins participating in ion-exchange in the cochlear lateral wall. Connexin 26 expression in the lateral wall may play a role in acquired hearing loss by maintaining the endocochlear potential and potassium concentration in the endolymph. We examined the effects of gentamicin on the expression of connexin 26 to obtain a better understanding of aminoglycosideototoxicity. METHODS: We detected changes in connexin 26 protein and mRNA expression in the cochlear lateral wall using immunohistochemistry staining, western blotting, and real-time PCR in rats after administration of a single dose of gentamicin. RESULTS: The expression of connexin 26 increased over time in type III fibrocytes after gentamicin administration. Elevated protein levels were detected 3 h after the single injection of gentamicin; while, mRNA levels increased after 24 h. CONCLUSION:Connexin 26 plays an important role in the acute effects of high-dose gentamicin and is probably involved in the pathogenesis of ototoxic deafness.
Authors: Vania A Figueroa; Mauricio A Retamal; Luis A Cea; José D Salas; Aníbal A Vargas; Christian A Verdugo; Oscar Jara; Agustín D Martínez; Juan C Sáez Journal: Front Cell Neurosci Date: 2014-09-04 Impact factor: 5.505