BACKGROUND: Runt-related transcription factor 2 (RUNX2) is a transcription factor that is closely related to bone formation, and prostate cancer (CaP) is the most common cancer to metastasize to bone. The present study investigated the expression levels of RUNX2 in human prostate tissue, and the correlation between RUNX2 levels and the clinicopathological characteristics of CaP. METHODS: A case-control study was conducted including 114 cases of newly diagnosed CaP and 114 age-matched BPH patients as controls. RUNX2 expression was estimated using real-time PCR and immunohistochemical staining. RESULTS: The mRNA expression of RUNX2 did not differ between CaP tissues and non-cancer BPH controls (P=0.825). However, RUNX2 expression was significantly decreased in patients with elevated PSA levels (≥20 ng ml(-1)), a Gleason score ≥8 and metastatic disease compared to those with low PSA, low Gleason score and non-metastatic disease (P=0.023, 0.005 and 0.014, respectively). Immunohistochemical analysis showed that 65.2% of the patients with positive RUNX2 nuclear staining had metastatic disease, which was present in only 25.9% of those with negative staining (P=0.010). CONCLUSIONS: RUNX2 mRNA expression was negatively correlated with CaP aggressiveness. Moreover, the nuclear location of RUNX2 may be a prognostic marker of metastasis in CaP.
BACKGROUND:Runt-related transcription factor 2 (RUNX2) is a transcription factor that is closely related to bone formation, and prostate cancer (CaP) is the most common cancer to metastasize to bone. The present study investigated the expression levels of RUNX2 in human prostate tissue, and the correlation between RUNX2 levels and the clinicopathological characteristics of CaP. METHODS: A case-control study was conducted including 114 cases of newly diagnosed CaP and 114 age-matched BPH patients as controls. RUNX2 expression was estimated using real-time PCR and immunohistochemical staining. RESULTS: The mRNA expression of RUNX2 did not differ between CaP tissues and non-cancer BPH controls (P=0.825). However, RUNX2 expression was significantly decreased in patients with elevated PSA levels (≥20 ng ml(-1)), a Gleason score ≥8 and metastatic disease compared to those with low PSA, low Gleason score and non-metastatic disease (P=0.023, 0.005 and 0.014, respectively). Immunohistochemical analysis showed that 65.2% of the patients with positive RUNX2 nuclear staining had metastatic disease, which was present in only 25.9% of those with negative staining (P=0.010). CONCLUSIONS:RUNX2 mRNA expression was negatively correlated with CaP aggressiveness. Moreover, the nuclear location of RUNX2 may be a prognostic marker of metastasis in CaP.
Authors: Moran Choe; Jessica L Brusgard; Saranya Chumsri; Lekhana Bhandary; Xianfeng Frank Zhao; Song Lu; Olga G Goloubeva; Brian M Polster; Gary M Fiskum; Geoffrey D Girnun; Myoung Sook Kim; Antonino Passaniti Journal: J Cell Biochem Date: 2015-10 Impact factor: 4.429
Authors: Xuhui Zhang; Jacqueline Akech; Gillian Browne; Stacey Russell; John J Wixted; Janet L Stein; Gary S Stein; Jane B Lian Journal: Int J Cancer Date: 2014-08-05 Impact factor: 7.396
Authors: Gillian H Little; Sanjeev K Baniwal; Helty Adisetiyo; Susan Groshen; Nyam-Osor Chimge; Sun Young Kim; Omar Khalid; Debra Hawes; Jeremy O Jones; Jacek Pinski; Dustin E Schones; Baruch Frenkel Journal: Cancer Res Date: 2014-03-19 Impact factor: 12.701
Authors: C Ge; G Zhao; Y Li; H Li; X Zhao; G Pannone; P Bufo; A Santoro; F Sanguedolce; S Tortorella; M Mattoni; S Papagerakis; E T Keller; R T Franceschi Journal: Oncogene Date: 2015-04-13 Impact factor: 9.867