BACKGROUND: SAGM is currently the standard additive solution used in Europe, while AS-3 is the third additive solution that has been licensed in the USA, and is also the one used in part of Canada. Although AS-3 is based on a saline-adenine-glucose solution, it also contains citrate and phosphate. Storage of red blood cell concentrates in CPD-SAGM is known to lead to the accumulation of a wide series of storage lesions, including membrane protein fragmentation and vesiculation, as we could previously determine through 2-dimensional gel electrophoresis. MATERIALS AND METHODS: Through 2D-SDS-IEF-polyacrilamide gel electrophoresis we performed a time course analysis (day 0, 21 and 42 of storage) of red blood cell membranes from leukocyte-filtered concentrates either stored in CPD-SAGM or CP2D-AS-3. RESULTS AND DISCUSSION: From the present study it emerges that the membrane protein profile of red blood cells stored in presence of AS-3 appears to be slightly different from (better than) previous reports on SAGM-stored counterparts. However, the increase of total membrane spot number due to the presence of fragments at day 21 and the significant decrease at day 42 are suggestive of a universal phenomenon which is not efficiently tackled by either of the two additive solutions investigated in the present study. CONCLUSION: To further delve into the storage lesion issue for RBCs stored in AS-3, it would be interesting in the future to assay metabolic changes over storage progression as well.
BACKGROUND:SAGM is currently the standard additive solution used in Europe, while AS-3 is the third additive solution that has been licensed in the USA, and is also the one used in part of Canada. Although AS-3 is based on a saline-adenine-glucose solution, it also contains citrate and phosphate. Storage of red blood cell concentrates in CPD-SAGM is known to lead to the accumulation of a wide series of storage lesions, including membrane protein fragmentation and vesiculation, as we could previously determine through 2-dimensional gel electrophoresis. MATERIALS AND METHODS: Through 2D-SDS-IEF-polyacrilamide gel electrophoresis we performed a time course analysis (day 0, 21 and 42 of storage) of red blood cell membranes from leukocyte-filtered concentrates either stored in CPD-SAGM or CP2D-AS-3. RESULTS AND DISCUSSION: From the present study it emerges that the membrane protein profile of red blood cells stored in presence of AS-3 appears to be slightly different from (better than) previous reports on SAGM-stored counterparts. However, the increase of total membrane spot number due to the presence of fragments at day 21 and the significant decrease at day 42 are suggestive of a universal phenomenon which is not efficiently tackled by either of the two additive solutions investigated in the present study. CONCLUSION: To further delve into the storage lesion issue for RBCs stored in AS-3, it would be interesting in the future to assay metabolic changes over storage progression as well.
Authors: Jacques Lacroix; Paul Hébert; Dean Fergusson; Alan Tinmouth; Morris A Blajchman; Jeannie Callum; Deborah Cook; John C Marshall; Lauralyn McIntyre; Alexis F Turgeon Journal: Transfus Med Rev Date: 2011-05-06
Authors: Marianna H Antonelou; Anastasios G Kriebardis; Konstantinos E Stamoulis; Effrosini Economou-Petersen; Lukas H Margaritis; Issidora S Papassideri Journal: Transfusion Date: 2009-10-23 Impact factor: 3.157
Authors: Tatsuro Yoshida; James P AuBuchon; Larry J Dumont; James D Gorham; Sean C Gifford; Kevin Y Foster; Mark W Bitensky Journal: Transfusion Date: 2008-07-08 Impact factor: 3.157
Authors: Angelo D'Alessandro; Travis Nemkov; Kaiqi Sun; Hong Liu; Anren Song; Andrew A Monte; Andrew W Subudhi; Andrew T Lovering; Daniel Dvorkin; Colleen G Julian; Christopher G Kevil; Gopi K Kolluru; Sruti Shiva; Mark T Gladwin; Yang Xia; Kirk C Hansen; Robert C Roach Journal: J Proteome Res Date: 2016-09-27 Impact factor: 4.466