CONTEXT: DNA damage following exposure to ultraviolet radiation (UVR) is important in skin cancer development. The predominant photoproduct, cyclobutane thymine dimer (T=T), is repaired and excreted in the urine, where it provides a biomarker of exposure. OBJECTIVE: To quantify urinary T=T levels after recreational sunlight exposure in adults and children. METHODS: Average UVR doses were measured with personal dosimeters. Urinary T=T was analysed with (32)P-postlabelling. RESULTS: Background levels of T=T increased significantly following exposure to sunlight. Amounts of T=T in urine of children and adults were not significantly different after adjusting for area of skin exposed and physiological differences. UVR dose and amounts of T=T correlated for both adults and children. CONCLUSION: Recreational exposure to sunlight in Sweden induces levels of DNA damage, clearly detectable in urine.
CONTEXT: DNA damage following exposure to ultraviolet radiation (UVR) is important in skin cancer development. The predominant photoproduct, cyclobutane thymine dimer (T=T), is repaired and excreted in the urine, where it provides a biomarker of exposure. OBJECTIVE: To quantify urinary T=T levels after recreational sunlight exposure in adults and children. METHODS: Average UVR doses were measured with personal dosimeters. Urinary T=T was analysed with (32)P-postlabelling. RESULTS: Background levels of T=T increased significantly following exposure to sunlight. Amounts of T=T in urine of children and adults were not significantly different after adjusting for area of skin exposed and physiological differences. UVR dose and amounts of T=T correlated for both adults and children. CONCLUSION: Recreational exposure to sunlight in Sweden induces levels of DNA damage, clearly detectable in urine.
Authors: Bibi Petersen; Hans C Wulf; Margarita Triguero-Mas; Peter A Philipsen; Elisabeth Thieden; Peter Olsen; Jakob Heydenreich; Payam Dadvand; Xavier Basagaña; Tove S Liljendahl; Graham I Harrison; Dan Segerbäck; Alois W Schmalwieser; Antony R Young; Mark J Nieuwenhuijsen Journal: J Invest Dermatol Date: 2014-05-20 Impact factor: 8.551
Authors: S J Felton; M S Cooke; R Kift; J L Berry; A R Webb; P M W Lam; F R de Gruijl; A Vail; L E Rhodes Journal: Br J Dermatol Date: 2016-11-18 Impact factor: 9.302