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Literature DB >> 228883

Studies on guinea-pig macrophage migration inhibitory factor (MIF). I. Glycoprotein nature and net charge.

Abstract

Guinea-pig macrophage migration inhibitory factor (MIF), obtained by the stimulation of sensitized lymph node cells with tuberculin PPD, was characterized as a glycoprotein by the following criteria: (a) its activity is destroyed by 0.02 M sodium periodate; (b) when MIF-containing culture fluids are subjected to precipitation by perchloric acid (final concentration 1 M), the inhibitory activity is recovered in the supernatant; and (c) MIF binds to Sepharose-linked concanavalin A and can be eluted with methyl-alpha-D-glucopyranoside. When MIF-containing culture supernatants are fractionated by isoelectrofocussing, migration inhibitory activity is recovered in a fraction with an isoelectric point of 4.4--4.6.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1979 PMID： 228883 PMCID： PMC1537770

Source DB: PubMed Journal: Clin Exp Immunol ISSN： 0009-9104 Impact factor: 4.330

25 in total

1. Partial purification of lymphokine activities by iso-electric focusing.

Authors: A Trudgett
Journal: J Immunol Methods Date: 1976 Impact factor: 2.303

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. The fractionation of antigen-dependent macrophage migration inhibition and macrophage activation factors from lymph draining a tuberculin reaction.

Authors: D M Lowe; P J Lachmann
Journal: Scand J Immunol Date: 1974 Impact factor: 3.487

4. Carcinoembryonic antigen: characterization and clinical applications.

Authors: W D Terry; P A Henkart; J E Coligan; C W Todd
Journal: Transplant Rev Date: 1974

Review 5. In vitro approaches to the mechanism of cell-mediated immune reactions.

Authors: B R Bloom
Journal: Adv Immunol Date: 1971 Impact factor: 3.543

6. Two migration inhibitory factors with different chromatographic behavior and isoelectric points.

Authors: H G Remold; A D Mednis
Journal: J Immunol Date: 1977-06 Impact factor: 5.422

7. Purification of antigen-dependent macrophage migration inhibition factor (MIF) from lymph draining a tuberculin reaction.

Authors: D M Scott; I McConnell; P Agomo; P J Lachmann
Journal: Immunology Date: 1978-04 Impact factor: 7.397

8. Studies on guinea-pig macrophage migration inhibitory factor (MIF). II. Purification of MIF after treatment with reducing and denaturing agents.

Authors: P Kotkes; E Pick
Journal: Clin Exp Immunol Date: 1979-09 Impact factor: 4.330

9. Studies on migration inhibitory factor (MIF): recovery of MIF activity after purification by gel filtration and disc electrophoresis.

Authors: H G Remold; A B Katz; E Haber; J R David
Journal: Cell Immunol Date: 1970-05 Impact factor: 4.868