Long-term follow-up of seven patients with ophthalmopathy not associated with thyroid autoimmunity: heterogeneity of autoimmune ophthalmopathy.

BACKGROUND: Ophthalmopathy is the most common extrathyroidal manifestation of Graves' disease. However, in approximately 5% of cases this autoimmune eye disorder occurs in the apparent absence of Graves' hyperthyroidism: the so-called euthyroid Graves' disease (EGD).
METHODS: Seven patients with EGD were followed for evidence of thyroid and orbital autoimmunity, for up to 10 years. Calsequestrin and collagen XIII antibodies were measured by enzyme linked immunosorbent assay (ELISA), and TSH-receptor (TSH-r) antibodies were measured as TSH-r-binding antibody (TRAb) and thyroid-stimulating immunoglobulin (TSI). Eye signs were characterized and quantified as clinical activity score (CAS), NOSPECS classes, Nunery types 1 and 2, and margin-reflex distance (MRD).
RESULTS: Calsequestrin antibodies were detected on at least one occasion in three of the seven patients and collagen XIII antibodies were detected one or more times in five patients. In one patient with isolated congestive ophthalmopathy who was studied intensely, collagen XIII antibodies were initially positive and then became negative as the eye disease stabilized, while antibodies targeting calsequestrin were always negative. TRAb was not detected in any patient, but TSI was detected in three patients on one occasion each. Ultrasound abnormalities were found in four of the six patients for whom this was carried out, but there was no clear evidence for thyroiditis in any of these patients. For comparison, 13 patients were studied with typical Graves' ophthalmopathy. There were no significant differences compared to EGD in respect to the prevalence of positive calsequestrin or collagen XIII antibodies, but these patients included more smokers (eight out of 13 versus none out of seven).
CONCLUSIONS: Earlier studies suggesting that patients with EGD eventually develop thyroid dysfunction have not been confirmed here, although follow-up continues, and the possibility that such patients have had thyroid autoimmunity in the past, or that they will develop it in the future cannot be excluded. Overall, it is likely that the ophthalmopathy associated with Graves' hyperthyroidism is the same disease as that observed in patients - such as those reported here - in whom thyroid dysfunction and thyroid autoimmunity are not present during the period of follow- up. The role of autoimmunity against the TSH-r in euthyroid patients with ophthalmopathy has not been proven and the significance of the orbital antibodies is unclear.

Ophthalmopathy is the most common extrathyroidal manifestation of Graves’ disease occurring in approximately 50% of patients.1–3 General, mild ophthalmopathy also occurs in a small proportion of patients with transient (subacute, silent) thyroiditis4 and in about one-third of patients with progressive (Hashimoto’s) thyroiditis.5 In approximately 5% of cases, ophthalmopathy occurs in the absence of Graves’ hyperthyroidism, where it is called EGD, even though this is an incorrect description of the disorder since these patients do not have, or do not develop over a 10-year period (as will be shown here), Graves’ hyperthyroidism. However, for the purpose of this study we will refer to these patients as having “EGD.”

The association between thyroid autoimmunity and ophthalmopathy is best explained by cross-reactivity against a thyroid- and orbital–tissue-shared antigen such as the TSH-r, which is expressed in the orbital preadipocyte6,7 and TSH-r antibodies are generally accepted as playing a role in the pathogenesis of thyroid-associated ophthalmopathy.8,9 However, their role in EGD is unclear; consequently, other possibilities must be considered.10

Several other autoantibodies are associated with ophthalmopathy; in particular, those targeting the calcium-binding skeletal muscle protein calsequestrin (CASQ1), which is localized to the sarcoplasmic reticulum of the skeletal muscle fibre11 and to the fibroblast cell membrane protein collagen XIII.12 Antibodies against CASQ1 are sensitive indicators of the ocular myopathic subtype of ophthalmopathy in patients with Graves disease,13,14 while those targeting collagen XIII are linked to the congestive ophthalmopathy subtype.15 Here, seven patients with EGD have been studied, including one patient with severe isolated congestive ophthalmopathy – who was studied intensively over a 6-year period – in whom collagen XIII antibodies were positive in the early stages, while calsequestrin antibodies were always negative and, for comparison, 13 patients with typical Graves’ ophthalmopathy were examined. The findings suggest that the ophthalmopathy of EGD and that associated with thyroid autoimmunity are the same disease, and in both cases are associated with other autoimmune disorders or markers.

Patients and methods

Clinical subjects

Seven patients with EGD, six females and one male, aged 30–63 years (mean age 51 years) were studied. Their demographic data, baseline thyroid function, and thyroid ultrasound findings are summarized in Table 1. Thyroid antibody testing results and details of any other personal or family history of autoimmunity are shown in Table 2. Three of the patients (patients 2, 4, and 5, Table 3), who had severe active disease, were being treated with steroids at the time of their first visit to the Thyroid Clinic and they all subsequently improved. The other four had never been treated with steroids or immunosuppressive drugs. For comparison, we studied 13 patients with typical “Graves’ ophthalmopathy” (ie, ophthalmopathy associated with Graves’ hyperthyroidism) matched for age, sex, ethnicity, and severity and activity of the eye disease with the seven patients with EGD. Their demographics and other details are shown in Table 5.

Table 1

Demographics, thyroid function, and thyroid ultrasound findings at the first visit in seven patients with euthyroid Graves’ disease

Patient number	Age/sex	Ethnicity	Thyroid function		Thyroid ultrasound	Histologya
			f T4	TSH
1	63/F	Caucasian	12.6	1.1	Normal
2	53/F	Malay	16	1.91	One large nodule, one small nodule, else normal	Follicular cell adenoma, no lymphocytes on FNA, or at thyroidectomy
3	50/F	Asian	9.9	0.84	Not performed	Nil
4	60/F	Asian	14	0.69	One small nodule, else normal	Nil
5	55/F	Caucasian	12.3	0.74	Normal	Nil
6	49/F	Caucasian	13	0.97	Several big nodules and overall colloid features	Colloid goiter, no lymphocytes at thyroidectomy
7	30/M	Lebanese	13.8	0.87	Enlarged, but normal texture	Nil

Notes:

assessed from fine needle aspiration (FNA) biopsy (patients 2, 6) or thyroidectomy (patient 2).

Abbreviations: fT4, free T4; TSH, thyroid-stimulating hormone.

Table 2

Thyroid antibodies, other autoantibodies, personal and family history of thyroid, and other autoimmune disorders in seven patients with euthyroid Graves’ disease

Patient number	Thyroid antibodiesa				Other autoantibodies		Personal history of thyroid or other autoimmunity	Known family history of thyroid or other autoimmunity
	TPO	Tg	TRAb	TSI	ANA	Other
1	<20	<20	<0.1	8.45	Neg	Fp	Nil	Hashimoto’s thyroiditis
	<20	<20	NT	19.38
	<20	<20	NT	NT
	<20	<20	NT	6.89
2	<20	<20	<0.1	17.64	Neg	Fp	Nil	Nil known
	<20	<20	NT	20.79
	<20	<20	NT	6.05
3	<20	<20	<0.1	13.71	Neg	NT	Nil	Rheumatoid arthritis
4	<20	<20	NT	NT	Neg	GPC	Vitiligo	Nil known
			NT	16.22
			NT	25.07
			NT	16.53
5	<20	<20	<0.1	13.16	Neg	NT	Nil	Nil known
6	<20	<20	<0.1	10.43	NT	NT	Nil	Nil known
7	<20	<20	NT	NT	NT	NT	Nil	Nil known

Notes:

Thyroid antibodies were measured in a commercial ELISA; TSH-receptor antibodies were measured as TSH-r-binding antibody (TRAb) (ref range < 2 IU/L) and as thyroid-stimulating immunoglobulin (TSI), (ref range 0%–19% B/B0). Results are shown for the first and subsequent clinic visits.

Abbreviations: ANA, antinuclear antibody; Fp, flavoprotein (the 64-kDa protein); GPC, gastric parietal cell; NT, not tested; Tg, thyroglobulin (cut off titer 20); TPO, thyroid peroxidase (cut off titer 20).

Table 3

Eye findings, smoking status, and treatment in seven patients with euthyroid Graves’ disease

Patient number	Duration of follow up (years)	Smokes	Eye signs				Orbital CT findings	Treatment
			NOSPECS classa	CAS	Nunery typeb	UERd
1	8	No	4	4	2	Yes	Enlarged EOM	Nil
2	10	No	4	4	2	No	Enlarged EOM	Prednisolone 25 mg
3	6	No	3	5	1	Yes	Normal EOM	Nil
4	8	No	2	3	1	No	Normal on two occasions 1 year apart	Prednisolone (50 mg/day) + steroid eye drops
5	7	No	4	1	2	No	EOM volumes increased	Prednisolone 7.5 mg
6	6	No	3	4	1	No	Minimal EOM swelling	Nil
7	3	No	3	2	1	Yes	Normal EOM, dilated ophthalmic vein	Nil

Notes:

NOSPECS classes of Werner18;

Nunery types 1 (ophthalmopathy without ocular myopathy) and 2 (with ocular myopathy)16;

Abbreviations: CAS, clinical activity score of Mourits et al17; EOM, extraocular muscles; UER, upper eyelid retraction.

Table 5

Eye findings, smoking status, and treatment at the time of the first clinic visit in 13 patients with Graves’ ophthalmopathy matched for age, sex, and ophthalmopathy severity, and activity with the patients with EGD

Patient number	Age/sex	Ethnicity	Duration of follow-up (years)	Personal history of thyroid or other autoimmunity	Known family history of thyroid or other autoimmunity	Smokes (Y/N)	TRAba	Eye signs				Orbital imaging findings	Thyroid antibodiesb	Treatment		Orbital antibodiesc
								NOSPECSd Class	CASe	Nunery typef	UERg		TPO/Tg	Eyes	Thyroid	CASQ1	CollXIII
1	38/F	Caucasian	6	Nil	Scleroderma	Y	NT	2	2	1	Yes	Not performed	Neg/neg	Nil	LT4	350	126
2	58	Caucasian	4	Nil	Premature grey hair	Y	>40	4	5	2	Yes	Not performed	100/neg	Nil	Carb	48	130
3	41	Filipino	5	Nil	Nil	N	NT	2	2	1	Yes	Not performed	Neg/neg	Nil	LT4	194	109
4	47/F	Caucasian	11/2	Nil	Nil	Y	9.2 (+)	4	4	2	Yes	Increased EOM volumes	1600/100	Prednisolone 10 mg	LT4	147	233
5	63/F	Caucasian	6	Nil	Nil	N	>40	4	4	2	Yes	Normal	400/400	Nil	Nil	261	204
6	76/F	Asian	2	Nil	Nil	N	2.9 (pos)	5	3	2	Yes	Increased EOM volumes	Neg/neg	Prednisolone 10 mg/day/Imuran	LT4	NT	NT
7	51/F	Caucasian	3	Celiac disease	Strong history of thyroid autoimmunity in many members of family	Y	?	4	4	2	Yes	Increased EOM volumes	Neg/neg	Prednisolone 5 mg/day/Imuran	LT4	39	1891
8	58/F	Caucasian	3	Sjögren’s disease	Graves’ disease, Graves’ ophthalmopathy	Y	NT	4	3	2	No	Not performed	400/neg	Nil	LT4	15	15
9	64/F	Caucasian	8	Nil	Nil	Y	6.4 (pos)	4	5	2	Yes	Increased EOM volumes	>1600/>1600	Nil at first visit, later selenium 100 μg bds	LT4	90	106
10	50/F	Caucasian	2	Nil	Nil	Y	5.3 (+)	3	2	1	No	Not performed	>25600, 1600	Nil	LT4	496	241
11	30/F	Caucasian	3	Nil	Nil	N	NT	2	2	1	No	Not performed		Nil	LT4	344	175
12	64/F	Caucasian	6	Psoriasis	Graves’ disease	Y	4.3 (pos)	4	5	2	Yes	Not performed	>1000, 91	Nil	LT5	11	110
13	44/M	Caucasian	2	Nil	Nil	N	>40	4	4	2	Yes	Not performed	Neg/neg	Nil	Carb	20	0

Notes:

thyroid antibodies were measured in a commercial ELISA; CAS, clinical activity score of Mourits et al17;

orbital antibodies were measured in a standard ELISA (see methods); positive tests are indicated in bold. A positive test was taken as an OD > the upper limit of normal for 30 healthy males aged <30; namely, 194 for CASQ1 and 174 for collagen XII;

NOSPECS classes of Werner18;;

nunery types 1 (ophthalmopathy without ocular myopathy) and 2 (with ocular myopathy)16.

Abbreviations: CAS, clinical activity score of Mourits et al17; CASQ1, skeletal muscle calsequestrin; CollXIII, collagen type XIII; EGD, enthyroid graves’ disease; NT, net tested; Tg, thyroglobulin (cut off titer 20); TRAB, TSH-r-binding antibody; TRAb, TSHr antibodies; TPO, thyroid peroxidase (cut off titer 20); UER, upper eyelid retraction.

Eye assessment

The grade, severity, and activity of the ophthalmopathy were classified as: (1) Nunery type 1 (without restrictive myopathy) or type 2 (with restrictive myopathy);16 (2) CAS (0–10) of Mourits et al,17 which is a measure of disease activity; (3) Werner’s NOSPECS classes;18 and (4) upper eyelid MRD, which is the distance between the center of the pupillary light reflex and the upper eyelid margin with the eye in primary gaze, as a measure of eyelid retraction; an MRD of >5 mm is taken as a significant upper eyelid retraction (UER). The degree of proptosis (mm) was measured using a Hertel exophthalmometer, where a positive reading was defined as ≥18 mm in either eye or >2 mm difference between the eyes.

Methods

Serum CASQ1 and collagen XIII antibodies were measured via a standard ELISA, as described in previous publications from this laboratory.4,5,13,14 Purified rabbit skeletal muscle calsequestrin, which has 97% homology with the human protein, was supplied by Dr Nicole Beard (ANU, Canberra, Australia) and recombinant human collagen XIII was provided by Drs Taina Pihlajaniemi and Tu Min (Oulu University, Finland). In preliminary studies, the assay was optimized in respect to antigen concentration and serum dilution as follows: 0.25 μg/mL for collagen XIII, and 0.5 μg/mL for CASQ1, and the optimal serum dilution was 1:25 for both antigens. The secondary antibody was a goat antihuman IgG at a dilution of 1 in 2000. A positive test was taken as an OD greater than the upper limit of normal for 30 healthy males aged <30 years; namely, 194 for CASQ1 and 174 for collagen XIII.

Other tests

Plasma-free thyroxin (fT4), and TSH and serum thyroglobulin (Tg) and thyroid peroxidise (TPO) antibodies were measured by Barratt and Smith Pathology (Sydney, Australia), using commercial kits according to the manufacturers’ instructions. TSH-r antibodies were measured as (1) TRAb, using a commercial kit according to the manufacturer’s instructions and (2) TSI, using fresh human thyroid cells as a source of human TSH-r and a commercial cAMP kit according to the manufacturer’s instructions (all kits were from Barratt and Smith Pathology). Local Ethical Committee approval was received for the study and the informed consent of the participating subjects was obtained for the extra blood taken for research.

Statistical analysis

Differences between the prevalence of smoking and positive serum calsequestrin and collagen XIII antibodies between patients with EGD and Graves’ ophthalmopathy were assessed using Fisher’s exact test (two-sided). A P value of <0.5 was taken as significant in all assessments.

Results

Thyroid antibodies

TPO and Tg antibodies were measured on several occasions in all seven patients (Table 2). Although the methodology changed over the period of follow-up, the tests were carried out on many occasions and were always clearly negative. Although not tested as frequently, TSH-r antibodies, measured as TRAb, were negative in all five of the patients tested, while TSI tests were borderline positive in patients 1 and 2, on one occasion each, and strongly positive in patient 4, 16 months after her first visit, but were otherwise negative (Table 2).

Thyroid ultrasonography

Thyroid ultrasound was carried out in six of the patients, of whom four had abnormalities; namely, a large suspicious nodule in patient 2, which was shown at thyroidectomy to be a follicular adenoma, one small benign nodule in patient 4, a multinodular goiter in patient 6, which was confirmed as colloid nodules from fine needle aspiration (FNA) biopsy, and an enlarged thyroid with normal texture and no nodules in patient 7. There was no evidence for thyroiditis in any of these patients at FNA (patients 4, 6) or thyroidectomy (patient 2) (Table 1).

Eye findings and orbital antibody levels

The nature and severity of the eye signs at the first visit to the Thyroid Clinic, Nepean Hospital, are summarized in Table 3. In all patients, the eye disease had been present for some time and was considered moderately severe or severe, with CAS ranging from 1 to 5 and NOSPECS classes from 2 to 4. The duration of the eye disease varied from 3–10 years. None of the patients were smokers. Orbital CT scans were carried out in all patients and showed definitely increased eye muscle volumes in three of them, all of whom had clinical eye muscle involvement (Nunery type 2, NOSPECS class 4). Three of the patients (patients 1, 3, 7) had UER. Three of the patients, all of whom had severe disease with eye muscle involvement (patients 2, 5) or severe congestive changes (patient 4, see below), were being treated with steroids at the time of their first visit.

Serum orbital antibodies were measured in all seven patients, in five of whom two or more tests were carried out (Table 4). Initial CASQ1 tests were positive in only one patient (patient 2) but collagen XIII antibodies were detected in three of the patients (patients 3, 4, 7) at their first visit. On follow-up testing, CASQ1 antibodies were detected in patient 1 at the most recent visit only, patient 2 at the fourth and fifth visits – associated with worsening of her eye signs (see below) – and in patient 5 at the second visit only. Collagen XIII antibodies were positive in one or more serum samples from five of the seven patients (Table 4).

Table 4

Correlations between parameters of the ophthalmopathy and serum levels of thyroid and orbital antibodies in seven patients with euthyroid Graves’ disease studied prospectively

		Ophthalmopathy			Orbital antibodiesa
		CAS	NOSPECS	Nuneryb	UER	CASQ1	CollXIII
1	9/05	4	4	2	No	32	36
	11/05	3	4	2	No	127	108
	7/07	3	2	2	Yes	92	97
	3/09	4	1	1	No	276	168
2	2/06	3	4	2	No	186	83
	5/06	4	3	1	No	160	50
	1/07	1	2	1	Yes	74	275
	1/08	1	2	1	Yes	372	1043
	7/08	2	3	1	No	897	1228
3	2/09	4	3	1	No	40	467
4	4/06	3	2	1	No	161	699
	12/06	3	2	1	No	105	203
	9/07	4	2	1	No	38	280
	10/07	3	2	1	No	55	106
	8/08	4	2	1	No	121	200
	10/08	4	2	1	No	153	207
5	6/07	1	4	2	No	32	98
	10/07	1	4	2	No	235	424
	9/10	1	4	2	No	172	942
6	6/09	4	4	2	No	0	163
	9/09	2	3	2	Yes	150	44
7	12/10	2	3	1	Yes	NTf	573

Notes:

Orbital antibodies were measured in a standard ELISA (see methods); positive tests are indicated in bold. A positive test was taken as an OD > the upper limit of normal for 30 healthy males aged <30; namely, 194 for CASQ1 and 174 for collagen XII;

NOSPECS classes of Werner18;

nunery types 1 (ophthalmopathy without ocular myopathy) and 2 (with ocular myopathy).16

Abbreviations: CAS, clinical activity score of Mourits et al17; CASQ1, skeletal muscle calsequestrin; CollXII, collagen type XII; UER, upper eyelid retraction; NT, not tested.

Patients 2, 4, and 7 were of special interest. Patient 2 initially had severe ophthalmopathy with eye muscle dysfunction (Nunery 2, CAS 3, NOSPECS class 4) but negative CASQ1 and collagen XIII antibody tests. The eye signs then improved over the next 2 years with steroid treatment (for 3 months) to Nunery 1, CAS 1, NOSPECS class 2, at which time both antibodies were positive, then worsened to CAS 2, NOSPECS class 3 (Nunery 1) as antibody tests became strongly positive (Table 4).

Patient 4 developed marked congestive ophthalmopathy in 2003, 3 years before she was first seen at the Thyroid Clinic, where she was studied in more detail. Throughout the study period her eyes had been variably itchy, gritty, watery, and swollen (CAS 3 or 4) with intermittent flareups requiring treatment with oral steroids, steroid eye drops, or both. At the time of her first clinic visit, she was being treated with prednisolone 50 mg/day, which was reduced over the next 3 months, and was then stopped. She had never complained of double vision or reduced vision, and her eye muscle function had always been normal. A CT scan of her orbits carried out in 2005 and 2006 showed normal eye muscle volumes. She had no upper eyelid lag, or UER, or proptosis at any stage. CASQ1 antibody tests were always negative, while collagen XIII antibodies were positive in the early samples, then negative or borderline positive in later samples (Table 4).

Patient 7, the only male in the group, who was first seen in December 2010, had marked UER of the right eye, slight chemosis of the right eye, and 2 mm of proptosis when compared to the left eye, but no signs in the other (left) eye. He had no eye pain or diplopia (NOSPECS class 3, Nunery 1, UER). He slept with the right eye open. An orbital MRI showed normal extra ocular muscles (EOM) but a dilated ophthalmic vein and increased cerebrospinal fluid around the optic nerve. An MRI of the arteries and veins revealed no evidence for an AV malformation and there was no evidence of an orbital tumor.

Except for patient 2 during the last 12 months of her follow- up where worsening congestive features were associated with high serum levels of both antibodies, and patient 4, in whom levels of collagen XIII antibodies correlated generally with the severity of her congestive signs, there was no significant correlation between levels of either antibody and the clinical features (Table 3).

Patients with Graves’ ophthalmopathy

For comparison, we studied a group of 13 patients with Graves’ ophthalmopathy, twelve females and one male, seen at the Thyroid Clinic over the same period of time and matched for age, sex, ethnicity, and duration and severity of their eye disease with the patients with EGD. Details of their ophthalmopathy and serum levels of thyroid and orbital antibodies at the time of the first clinic visit and subsequently (orbital antibodies only) are summarized in Table 5. Their thyroid status was variable at the time of their first clinic visit depending on the stage of their disease and its treatment and thyroid antibodies were usually strongly positive. TSH-r antibodies were initially positive in all seven patients in whom this test was carried out. Three of the patients (patients 4, 6, 7) were being treated with prednisolone, alone, or with Imuran (two patients), at the time of their first clinic visit. Compared to the patients with EGD, the main differences were: (1) there were more smokers (eight out of 13 versus none out of seven, two-sided Fisher’s exact test, P < 0.05); (2) their thyroid glands were diffusely enlarged with increased vascularity, as expected, and a few had benign thyroid nodules on real-time ultrasonography; (3) calsequestrin antibodies were initially detected in five out of the 13 (38%) patients in whom this test was performed, at the first clinic visit, while tests were always negative in the other six patients (results not shown) [compared to one out of seven (15%) and four out of seven (67%), respectively, patients with EGD (two-sided Fisher’s exact test, P = NS, P = NS, respectively)] and; (4) collagen XIII antibodies were detected in five out of the 13 (38%) patients in whom this test was performed, at the first clinic visit, and in another two patients at some time during their course (results not shown) (overall 75% of patients) [compared to one out of 7 (14%) and 5 out of 7 (71%), respectively, EGD patients (two-sided Fisher’s exact test, P = NS, P = NS, respectively)].

Discussion

That the ophthalmopathy of Graves’ disease can occur in the apparent absence of hyperthyroidism or hypothyroidism is well known, but it is not clear whether the two disorders have the same pathogenesis. Here, we report seven patients with “EGD” who were studied over a 10-year period and who have not developed Hashimoto’s thyroiditis or Graves’ disease as determined from thyroid function tests, thyroid antibody testing and, where available, histological examination of their thyroid tissue at thyroidectomy or FNA biopsy. Some of the patients had a personal or family history of other autoimmune disorders or autoantibodies, suggesting that “autoimmune ophthalmopathy” can be considered one component of a limited, multisystem autoimmune disorder, as previously postulated.19,20

Serum calsequestrin and collagen XIII antibodies were measured in these patients and real-time thyroid ultrasonographic findings are reported. Included in these seven patients was a patient with severe congestive ophthalmopathy, but who had no evidence for eye muscle damage, in whom collagen XIII antibodies were initially positive and then became negative and in whom antibodies targeting calsequestrin were always negative. We believe that this case study supports the role of autoimmunity against collagen XIII in the pathogenesis of a putative congestive ophthalmopathy subtype of thyroid eye disease.

In an earlier study,21 we found that all patients with EGD who were followed for up to 3 years developed clinical or immunological evidence for either Hashimoto’s thyroiditis or Graves’ disease. Then, in a study where we additionally tested for cytotoxicity against thyroid and eye muscle cells in patients with EGD, over half of a group of patients with EGD showed evidence of thyroid autoimmunity.22 However, most of these latter patients would now have been classified as having Hashimoto’s thyroiditis with upper eyelid disease, rather than as having “EGD.”

For comparison, a group of 13 age-, sex-, and ethnicity-matched patients with typical Graves’ ophthalmopathy who attended the thyroid clinic over the same period of time, and who had similar eye disease in respect of activity and severity were studied. Overall, there were no significant differences between the two groups in respect to the prevalence of calsequestrin and collagen XII antibodies, but there were significantly more smokers among the Graves’ ophthalmopathy patients, the significance of which is unclear. This suggests that the ophthalmopathy is the same whether it is associated with thyroid autoimmunity or not, and that it is one feature of an autoimmune cluster that includes thyroid autoimmunity and inflammation of the bones, skin, and lacrimal glands.

Collagen XIII is a fibroblast cell membrane protein and serum antibodies against collagen XIII have been linked to recent onset, congestive ophthalmopathy in patients with Graves’ ophthalmopathy.14,15 Five of our patients had detectable collagen XIII antibodies, including patient 4, in whom serum levels correlated generally with the clinical severity of her eye signs and symptoms. Since collagen XIII is expressed in the orbital fibroblast cell membranes12 its targeting by collagen XIII antibodies might lead to fibroblast stimulation, fibrosis, and glycosaminoglycans overproduction – well recognized features of Graves’ ophthalmopathy.23,24

The TSH-r is expressed in the orbital preadipocytes and fibroblasts and many researchers propose that its targeting there leads to the development of ophthalmopathy.2,7,9 However, in patient 4 with severe isolated congestive ophthalmopathy, TSH-r antibodies were always negative, except for one strongly positive test, as TSI, on one occasion, and the overall prevalence of these antibodies was lower than in patients with Graves’ ophthalmopathy. It is therefore tempting to conclude that these antibodies play no major role in the pathogenesis of this “autoimmune ophthalmopathy.” However, TSH-r antibodies might have been present before the onset of ophthalmopathy in patients without associated thyroid disease, but not tested for. In order to elucidate the significance of TSH-r antibodies, a long-term study of patients with recent onset ophthalmopathy with and without thyroid autoimmunity in which the TSH-r-binding–blocking antibodies that have been implicated in the pathogenesis of Graves’ ophthalmopathy,25 other TSH-r antibodies, as well as calsequestrin, Fp, and collagen XIII antibodies are measured, needs to be carried out.

The major calcium-binding protein, CASQ1, seems to be an important target of the autoimmune reactions of the eye muscle component of the ophthalmopathy associated with Graves’ hyperthyroidism (“Graves ophthalmopathy”)26,27 and of the upper eyelid muscle reaction of Hashimoto’s thyroiditis. 5 CASQ1 is expressed 4.7 times more in eye muscle than in other skeletal muscle,28 which could partly explain the localization of skeletal muscle inflammation in the orbit and upper eyelids in these patients.

Finally, although out of the scope of the present study, the role of cytokines and other chemokines in the pathogenesis of ophthalmopathy is also likely to be important.29,30 It is interesting to speculate that the profile of these mediators may differ in patients with “EGD” and those with typical Graves’ ophthalmopathy, that is, where there is associated thyroid autoimmunity. The role of cytokines and T-cell autoimmunity can be studied in an animal model for “autoimmune ophthalmopathy,” which we are currently developing.

In conclusion, earlier studies suggesting that patients with EGD eventually develop thyroid dysfunction have not been confirmed here, although the possibility that patients with “EGD” had thyroid autoimmunity in the past, or that they will develop it in the future, cannot be excluded. Overall, it is likely that the ophthalmopathy associated with Graves’ hyperthyroidism (“Graves’ ophthalmopathy”) is the same disease as that observed in patients, such as those reported here, in whom thyroid dysfunction and thyroid autoimmunity are not present initially, nor where such dysfunction and autoimmunity do not develop during the period of follow-up that we have called “EGD”. In both cases, there is an association with other autoimmune disorders or markers. As the role of autoimmunity against the TSH-r in ophthalmopathy has not been proven, and because the significance of orbital antibodies is unclear, additional pathogenetic mechanisms for this autoimmune eye disorder need to be considered.