BACKGROUND: Cereals used for beer manufacturing contain gluten, which is immunotoxic for celiac patients. The gluten remaining after processes of malting and brewing is mostly hydrolyzed, which makes practical evaluation of the immunotoxicity of the gluten pools challenging. RESULTS: We analyzed the presence of gluten peptides equivalent to the major immunotoxic protease-resistant gliadin 33-mer in 100 Belgium beers, using monoclonal antibodies (G12/A1). Immunochromatographic strips and enzyme-linked immonosorbent assay G12/A1 methods estimated at least 20 ppm gluten equivalents in 90 beers and gluten-free in 10 beers. The G12/A1 reactivity of beer high-performance liquid chromatographic fractions correlated to the presence of T-cell-reactive epitopes identified by peptide sequencing. CONCLUSION: The determination of equivalent gliadin 33-mer epitopes in beers has been shown to be practical, specific, and sensitive for the measurement of potential immunotoxicity for celiac patients.
BACKGROUND: Cereals used for beer manufacturing contain gluten, which is immunotoxic for celiac patients. The gluten remaining after processes of malting and brewing is mostly hydrolyzed, which makes practical evaluation of the immunotoxicity of the gluten pools challenging. RESULTS: We analyzed the presence of gluten peptides equivalent to the major immunotoxic protease-resistant gliadin 33-mer in 100 Belgium beers, using monoclonal antibodies (G12/A1). Immunochromatographic strips and enzyme-linked immonosorbent assay G12/A1 methods estimated at least 20 ppm gluten equivalents in 90 beers and gluten-free in 10 beers. The G12/A1 reactivity of beer high-performance liquid chromatographic fractions correlated to the presence of T-cell-reactive epitopes identified by peptide sequencing. CONCLUSION: The determination of equivalent gliadin 33-mer epitopes in beers has been shown to be practical, specific, and sensitive for the measurement of potential immunotoxicity for celiac patients.
Authors: Isabel Comino; Fernando Fernández-Bañares; María Esteve; Luís Ortigosa; Gemma Castillejo; Blanca Fambuena; Carmen Ribes-Koninckx; Carlos Sierra; Alfonso Rodríguez-Herrera; José Carlos Salazar; Ángel Caunedo; J M Marugán-Miguelsanz; José Antonio Garrote; Santiago Vivas; Oreste Lo Iacono; Alejandro Nuñez; Luis Vaquero; Ana María Vegas; Laura Crespo; Luis Fernández-Salazar; Eduardo Arranz; Victoria Alejandra Jiménez-García; Marco Antonio Montes-Cano; Beatriz Espín; Ana Galera; Justo Valverde; Francisco José Girón; Miguel Bolonio; Antonio Millán; Francesc Martínez Cerezo; César Guajardo; José Ramón Alberto; Mercé Rosinach; Verónica Segura; Francisco León; Jorge Marinich; Alba Muñoz-Suano; Manuel Romero-Gómez; Ángel Cebolla; Carolina Sousa Journal: Am J Gastroenterol Date: 2016-09-20 Impact factor: 10.864
Authors: Ana Real; Isabel Comino; Ma de Lourdes Moreno; Miguel Ángel López-Casado; Pedro Lorite; Ma Isabel Torres; Ángel Cebolla; Carolina Sousa Journal: PLoS One Date: 2014-06-25 Impact factor: 3.240
Authors: María de Lourdes Moreno; Ángel Cebolla; Alba Muñoz-Suano; Carolina Carrillo-Carrion; Isabel Comino; Ángeles Pizarro; Francisco León; Alfonso Rodríguez-Herrera; Carolina Sousa Journal: Gut Date: 2015-11-25 Impact factor: 23.059