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Literature DB >> 22886271

Site-specific labeling of his-tagged Nanobodies with ⁹⁹mTc: a practical guide.

Catarina Xavier¹, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D'Huyvetter, Jens De Vos, Sam Massa, Tony Lahoutte, Vicky Caveliers.

Abstract

99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.

Entities: Chemical

Mesh：

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Year: 2012 PMID： 22886271 DOI： 10.1007/978-1-61779-968-6_30

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

16 in total

1. Specific targeting of atherosclerotic plaques in ApoE(-/-) mice using a new Camelid sdAb binding the vulnerable plaque marker LOX-1.

Authors: Jens De Vos; Iris Mathijs; Catarina Xavier; Sam Massa; Ulrich Wernery; Luc Bouwens; Tony Lahoutte; Serge Muyldermans; Nick Devoogdt
Journal: Mol Imaging Biol Date: 2014-10 Impact factor: 3.488

2. Evaluation of [^99mTc]Radiolabeled Macrophage Mannose Receptor-Specific Nanobodies for Targeting of Atherosclerotic Lesions in Mice.

Authors: Gezim Bala; Henri Baudhuin; Isabel Remory; Kris Gillis; Pieterjan Debie; Ahmet Krasniqi; Tony Lahoutte; Geert Raes; Nick Devoogdt; Bernard Cosyns; Sophie Hernot
Journal: Mol Imaging Biol Date: 2018-04 Impact factor: 3.488

3. Molecular Imaging with Kupffer Cell-Targeting Nanobodies for Diagnosis and Prognosis in Mouse Models of Liver Pathogenesis.

Authors: Fang Zheng; Amanda Sparkes; Patrick De Baetselier; Steve Schoonooghe; Benoit Stijlemans; Serge Muyldermans; Véronique Flamand; Jo A Van Ginderachter; Nick Devoogdt; Geert Raes; Alain Beschin
Journal: Mol Imaging Biol Date: 2017-02 Impact factor: 3.488

Review 4. Nanobodies for Medical Imaging: About Ready for Prime Time?

Authors: Léa Berland; Lauren Kim; Omar Abousaway; Andrea Mines; Shruti Mishra; Louise Clark; Paul Hofman; Mohammad Rashidian
Journal: Biomolecules Date: 2021-04-26

5. A Fusion Protein of RGD4C and β-Lactamase Has a Favorable Targeting Effect in Its Use in Antibody Directed Enzyme Prodrug Therapy.

Authors: Hao Wang; Xiao-Liang Zhou; Wei Long; Jin-Jian Liu; Fei-Yue Fan
Journal: Int J Mol Sci Date: 2015-04-28 Impact factor: 5.923

6. Specificity Evaluation and Disease Monitoring in Arthritis Imaging with Complement Receptor of the Ig superfamily targeting Nanobodies.

Authors: Fang Zheng; Harris Perlman; Patrick Matthys; Yurong Wen; Tony Lahoutte; Serge Muyldermans; Shemin Lu; Patrick De Baetselier; Steve Schoonooghe; Nick Devoogdt; Geert Raes
Journal: Sci Rep Date: 2016-10-25 Impact factor: 4.379

Site-specific labeling of his-tagged Nanobodies with ⁹⁹mTc: a practical guide.

1. Specific targeting of atherosclerotic plaques in ApoE(-/-) mice using a new Camelid sdAb binding the vulnerable plaque marker LOX-1.

2. Evaluation of [^99mTc]Radiolabeled Macrophage Mannose Receptor-Specific Nanobodies for Targeting of Atherosclerotic Lesions in Mice.

3. Molecular Imaging with Kupffer Cell-Targeting Nanobodies for Diagnosis and Prognosis in Mouse Models of Liver Pathogenesis.

Review 4. Nanobodies for Medical Imaging: About Ready for Prime Time?

5. A Fusion Protein of RGD4C and β-Lactamase Has a Favorable Targeting Effect in Its Use in Antibody Directed Enzyme Prodrug Therapy.

6. Specificity Evaluation and Disease Monitoring in Arthritis Imaging with Complement Receptor of the Ig superfamily targeting Nanobodies.

7. Non-invasive assessment of murine PD-L1 levels in syngeneic tumor models by nuclear imaging with nanobody tracers.

8. A nanobody targeting carcinoembryonic antigen as a promising molecular probe for non-small cell lung cancer.

9. Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use.

10. Identification of Nanobodies against the Acute Myeloid Leukemia Marker CD33.