OBJECTIVES: To investigate the expression of TLR-4 (toll-like receptor) on human cervical cancer and find the biological function of the TLR-4 signal system. METHODS: The immunohistochemistry method was performed to study the protein expression and distribution of TLR-4. The viability of HeLa cells was determined by cell viability assay. Cell proliferation was detected by FCM, ELISA and Western blot were used to observe the gene and protein expression of IL-6 and TGF-beta1 in Hela cell lines. RESULTS: TLR-4 was over-expressed in cervix cancer, and its activation by LPS promotes proliferation and anti-apoptosis in Hela cells in vitro. Moreover the cell line proliferation increased in a dose- and time-dependent manner. The production of IL-6 and TGF-beta1 were promoted through the activation of the NF-kappaB signaling pathway.
OBJECTIVES: To investigate the expression of TLR-4 (toll-like receptor) on human cervical cancer and find the biological function of the TLR-4 signal system. METHODS: The immunohistochemistry method was performed to study the protein expression and distribution of TLR-4. The viability of HeLa cells was determined by cell viability assay. Cell proliferation was detected by FCM, ELISA and Western blot were used to observe the gene and protein expression of IL-6 and TGF-beta1 in Hela cell lines. RESULTS:TLR-4 was over-expressed in cervix cancer, and its activation by LPS promotes proliferation and anti-apoptosis in Hela cells in vitro. Moreover the cell line proliferation increased in a dose- and time-dependent manner. The production of IL-6 and TGF-beta1 were promoted through the activation of the NF-kappaB signaling pathway.
Authors: Xiao Yang; Gan Tao Chen; Yan Qing Wang; Shu Xian; Li Zhang; Shao Ming Zhu; Feng Pan; Yan Xiang Cheng Journal: Mol Med Rep Date: 2017-11-20 Impact factor: 2.952
Authors: Marconi Rego Barros; Talita Helena Araújo de Oliveira; Cristiane Moutinho Lagos de Melo; Aldo Venuti; Antonio Carlos de Freitas Journal: J Immunol Res Date: 2018-05-02 Impact factor: 4.818