Literature DB >> 22868107

A defensin-like peptide from Phaseolus vulgaris cv. 'King Pole Bean'.

Jack H Wong1, David C W Ip, T B Ng, Y S Chan, F Fang, W L Pan.   

Abstract

A 5447 Da antifungal peptide with an N-terminal sequence highly homologous to plant defensins was purified from Phaseolus vulgaris cv. 'King Pole Bean' by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75. The isolated peptide inhibited growth of a number of fungal species, including Mycosphaerella arachidicola, Saccharomyces cerevisiae and Candida albicans, with IC(50) values of 3.9, 4.0 and 8.4 μM, respectively. Using the membrane non-permeable DNA-binding dye SYTOX green, it was found that the peptide increased the cell membrane permeability of M. arachidicola, S. cerevisiae and C. albicans.
Copyright © 2012 Elsevier Ltd. All rights reserved.

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Year:  2012        PMID： 22868107     DOI： 10.1016/j.foodchem.2012.04.119

Source DB:  PubMed          Journal:  Food Chem        ISSN： 0308-8146            Impact factor:   7.514


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