Literature DB >> 2286630

Analysis of dilute peptide samples by capillary zone electrophoresis.

R Aebersold1, H D Morrison.   

Abstract

We report a method for the analysis of dilute peptide solutions by capillary zone electrophoresis. The procedure is based on an electrophoretic concentration step of the applied peptide solution in the capillary (stacking) prior to separation, thus allowing the application of increased sample volumes without a breakdown in resolution. Given a constant configuration of the hardware, the method permits the analysis of peptide solutions of an at least 5 times lower concentration than previously possible. The method was applied to the direct analysis of peptide samples separated by narrow-bore reversed-phase high-performance liquid chromatography for high-sensitivity peptide-sequence analysis.

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Year:  1990        PMID： 2286630     DOI： 10.1016/s0021-9673(01)90206-7

Source DB:  PubMed          Journal:  J Chromatogr


  14 in total

Review 1.  On-line preconcentration methods for capillary electrophoresis.

Authors:  D M Osbourn; D J Weiss; C E Lunte
Journal:  Electrophoresis       Date:  2000-08       Impact factor: 3.535

2.  Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Phosphoproteomics with the Production of over 11,000 Phosphopeptides from the Colon Carcinoma HCT116 Cell Line.

Authors:  Daoyang Chen; Katelyn R Ludwig; Oleg V Krokhin; Vic Spicer; Zhichang Yang; Xiaojing Shen; Amanda B Hummon; Liangliang Sun
Journal:  Anal Chem       Date:  2019-01-23       Impact factor: 6.986

3.  Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry.

Authors:  Elijah N McCool; Rachele Lubeckyj; Xiaojing Shen; Qiang Kou; Xiaowen Liu; Liangliang Sun
Journal:  J Vis Exp       Date:  2018-10-24       Impact factor: 1.355

4.  Single-Shot Capillary Zone Electrophoresis-Tandem Mass Spectrometry Produces over 4400 Phosphopeptide Identifications from a 220 ng Sample.

Authors:  Zhenbin Zhang; Alexander S Hebert; Michael S Westphall; Joshua J Coon; Norman J Dovichi
Journal:  J Proteome Res       Date:  2019-06-26       Impact factor: 4.466

Review 5.  Applications of capillary electrophoresis in pharmaceutical analysis.

Authors:  S R Rabel; J F Stobaugh
Journal:  Pharm Res       Date:  1993-02       Impact factor: 4.200

6.  Capillary Zone Electrophoresis-Tandem Mass Spectrometry with Activated Ion Electron Transfer Dissociation for Large-scale Top-down Proteomics.

Authors:  Elijah N McCool; Jean M Lodge; Abdul Rehman Basharat; Xiaowen Liu; Joshua J Coon; Liangliang Sun
Journal:  J Am Soc Mass Spectrom       Date:  2019-05-09       Impact factor: 3.109

7.  Single-Shot Top-Down Proteomics with Capillary Zone Electrophoresis-Electrospray Ionization-Tandem Mass Spectrometry for Identification of Nearly 600 Escherichia coli Proteoforms.

Authors:  Rachele A Lubeckyj; Elijah N McCool; Xiaojing Shen; Qiang Kou; Xiaowen Liu; Liangliang Sun
Journal:  Anal Chem       Date:  2017-11-07       Impact factor: 6.986

Review 8.  Identification and Quantification of Proteoforms by Mass Spectrometry.

Authors:  Leah V Schaffer; Robert J Millikin; Rachel M Miller; Lissa C Anderson; Ryan T Fellers; Ying Ge; Neil L Kelleher; Richard D LeDuc; Xiaowen Liu; Samuel H Payne; Liangliang Sun; Paul M Thomas; Trisha Tucholski; Zhe Wang; Si Wu; Zhijie Wu; Dahang Yu; Michael R Shortreed; Lloyd M Smith
Journal:  Proteomics       Date:  2019-05       Impact factor: 3.984

Review 9.  Dynamic pH junction preconcentration in capillary electrophoresis- electrospray ionization-mass spectrometry for proteomics analysis.

Authors:  Guijie Zhu; Liangliang Sun; Norman J Dovichi
Journal:  Analyst       Date:  2016-07-27       Impact factor: 4.616

10.  Coupling Capillary Zone Electrophoresis to a Q Exactive HF Mass Spectrometer for Top-down Proteomics: 580 Proteoform Identifications from Yeast.

Authors:  Yimeng Zhao; Liangliang Sun; Guijie Zhu; Norman J Dovichi
Journal:  J Proteome Res       Date:  2016-08-25       Impact factor: 4.466
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