BACKGROUND: To evaluate the accuracy and precision of a method for serum aldosterone using supported liquid extraction (SLE) for sample preparation instead of the more conventional liquid-liquid extraction (LLE) approach. METHODS: Two independently developed SLE-based LC-MS/MS methods for serum aldosterone (sample volumes 250 μl and 300 μl respectively) were compared to a modification of a previously reported LLE approach (sample volume 500 μl) in two method comparisons (n=75 and n=97). SLE analyses were performed at two separate centres. Precision was evaluated at a single site using human pools in head-to-head comparison between SLE and LLE. All analyses were performed on the ABSCIEX API-5000 LC-MS/MS system. RESULTS: At four increasing pool concentrations spanning 67-1060 pmol/l, total precision for SLE ranged from 6.8-4.1% compared with 11.1-4.3% for LLE. Differences did not reach statistical significance except at the lowest concentration where SLE was superior. Pasing Bablok regression comparisons were SLE=0.96×LLE-5.8 pmol/l (R(2)=0.985) and SLE=0.96×LLE-0.44 pmol/l (R(2)=0.969). CONCLUSIONS: For analysis of serum aldosterone on the ABSCIEX API-5000, SLE affords a smaller sample volume while maintaining the accuracy and precision performance of LLE. By avoiding specimen vortexing, SLE also allows for greater automation in the sample preparation.
BACKGROUND: To evaluate the accuracy and precision of a method for serum aldosterone using supported liquid extraction (SLE) for sample preparation instead of the more conventional liquid-liquid extraction (LLE) approach. METHODS: Two independently developed SLE-based LC-MS/MS methods for serum aldosterone (sample volumes 250 μl and 300 μl respectively) were compared to a modification of a previously reported LLE approach (sample volume 500 μl) in two method comparisons (n=75 and n=97). SLE analyses were performed at two separate centres. Precision was evaluated at a single site using human pools in head-to-head comparison between SLE and LLE. All analyses were performed on the ABSCIEX API-5000 LC-MS/MS system. RESULTS: At four increasing pool concentrations spanning 67-1060 pmol/l, total precision for SLE ranged from 6.8-4.1% compared with 11.1-4.3% for LLE. Differences did not reach statistical significance except at the lowest concentration where SLE was superior. Pasing Bablok regression comparisons were SLE=0.96×LLE-5.8 pmol/l (R(2)=0.985) and SLE=0.96×LLE-0.44 pmol/l (R(2)=0.969). CONCLUSIONS: For analysis of serum aldosterone on the ABSCIEX API-5000, SLE affords a smaller sample volume while maintaining the accuracy and precision performance of LLE. By avoiding specimen vortexing, SLE also allows for greater automation in the sample preparation.
Authors: Suzanne P Stam; Annet Vulto; Michel J Vos; Michiel N Kerstens; Abraham Rutgers; Ido Kema; Daan J Touw; Stephan Jl Bakker; André P van Beek Journal: BMJ Open Date: 2022-04-26 Impact factor: 3.006
Authors: Annet Vulto; Martijn van Faassen; Michiel N Kerstens; André P van Beek Journal: Front Endocrinol (Lausanne) Date: 2022-04-20 Impact factor: 6.055