PURPOSE: Several culture media are available to be used in ART. However it is uncertain whether embryos would preferably benefit from one type of medium or the association of different media. METHODS: We performed this study to evaluate the impact of simultaneous transfer of embryos independently cultured in two distinct culture media, on pregnancy outcome. A total of 722 couples who underwent infertility treatment were sequentially allocated into three groups: those who had half of the embryos individually cultured in MEM and the other half cultured in sequential media (MEM + Seq Group) (n = 243); those who had all embryos cultured only in sequential medium (Seq Group) (n = 239); and those who had all embryos cultured only in MEM (MEM Group) (n = 240). RESULTS: The pregnancy rate was higher in the MEM + Seq group (51.8 %) than the Seq group (36.7 %) (p < 0.001). However the pregnancy rate observed in the MEM group was similar to the others (44.2 %). When a logistic regression test was applied it demonstrated that the number of transferred embryos did not interfere in the pregnancy rates. CONCLUSIONS: Our results suggests that offering different culture conditions for sibling embryos with subsequent transfer of embryos that were kept in distinct culture media, might increase pregnancy rates in assisted reproduction cycles.
PURPOSE: Several culture media are available to be used in ART. However it is uncertain whether embryos would preferably benefit from one type of medium or the association of different media. METHODS: We performed this study to evaluate the impact of simultaneous transfer of embryos independently cultured in two distinct culture media, on pregnancy outcome. A total of 722 couples who underwent infertility treatment were sequentially allocated into three groups: those who had half of the embryos individually cultured in MEM and the other half cultured in sequential media (MEM + Seq Group) (n = 243); those who had all embryos cultured only in sequential medium (Seq Group) (n = 239); and those who had all embryos cultured only in MEM (MEM Group) (n = 240). RESULTS: The pregnancy rate was higher in the MEM + Seq group (51.8 %) than the Seq group (36.7 %) (p < 0.001). However the pregnancy rate observed in the MEM group was similar to the others (44.2 %). When a logistic regression test was applied it demonstrated that the number of transferred embryos did not interfere in the pregnancy rates. CONCLUSIONS: Our results suggests that offering different culture conditions for sibling embryos with subsequent transfer of embryos that were kept in distinct culture media, might increase pregnancy rates in assisted reproduction cycles.
Authors: Lora K Shahine; Amin A Milki; Lynn M Westphal; Valerie L Baker; Barry Behr; Ruth B Lathi Journal: Fertil Steril Date: 2010-09-01 Impact factor: 7.329
Authors: Simon Cooke; Patrick Quinn; Lee Kime; Cheryl Ayres; John P P Tyler; Geoff L Driscoll Journal: Fertil Steril Date: 2002-12 Impact factor: 7.329
Authors: N S Macklon; M H E C Pieters; M A Hassan; P H M Jeucken; M J C Eijkemans; B C J M Fauser Journal: Hum Reprod Date: 2002-10 Impact factor: 6.918
Authors: Iratxe López-Pelayo; Javier María Gutiérrez-Romero; Ana Isabel Mangano Armada; María Mercedes Calero-Ruiz; Pablo Javier Moreno de Acevedo-Yagüe Journal: JBRA Assist Reprod Date: 2018-06-01