OBJECTIVE: To study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase. METHOD: EtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques. RESULT: Nine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase. CONCLUSION: All compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.
OBJECTIVE: To study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase. METHOD:EtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques. RESULT: Nine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase. CONCLUSION: All compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.
Authors: Cynthia A Adinortey; Gabriel B Kwarko; Russell Koranteng; Daniel Boison; Issaka Obuaba; Michael D Wilson; Samuel K Kwofie Journal: Curr Issues Mol Biol Date: 2022-02-21 Impact factor: 2.976