Literature DB >> 22855519

Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue.

Scott A Koepsell1, Steven H Hinrichs, Peter C Iwen.   

Abstract

A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.

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Year:  2012        PMID： 22855519      PMCID： PMC3457455          DOI： 10.1128/JCM.01705-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

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3.  Improving molecular detection of fungal DNA in formalin-fixed paraffin-embedded tissues: comparison of five tissue DNA extraction methods using panfungal PCR.

Authors:  C Muñoz-Cadavid; S Rudd; S R Zaki; M Patel; S A Moser; M E Brandt; B L Gómez
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Review 4.  Histoplasmosis: a clinical and laboratory update.

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5.  PCR detection of Histoplasma capsulatum var. capsulatum in whole blood of a renal transplant patient with disseminated histoplasmosis.

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8.  Detection of Histoplasma capsulatum DNA in human samples by real-time polymerase chain reaction.

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4.  Detection of Histoplasma capsulatum in Organic Fertilizers by Hc100 Nested Polymerase Chain Reaction and Its Correlation with the Physicochemical and Microbiological Characteristics of the Samples.

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Review 6.  Diagnosis of histoplasmosis: current status and perspectives.

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7.  Endemic mycoses in immunocompromised hosts.

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8.  Histoplasma capsulatum endocarditis: multicenter case series with review of current diagnostic techniques and treatment.

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9.  Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase.

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Review 10.  Detection of microorganisms in granulomas that have been formalin-fixed: review of the literature regarding use of molecular methods.

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