[Effect of protoplast formation and regeneration on the production of nebramycin complex in Streptomyces cremeus subsp. tobramycini].

Production of the nebramycin complex in Streptomyces cremeus subsp. tobramycini before and after the protoplast formation and regeneration was comparatively studied. The antibiotic production was estimated by the total activity and component composition of the nebramycin complex. It was found that formation and regeneration of the protoplast led to lowering of the activity and changing of the complex component composition. Strains mainly synthesizing each one of the three basic components of the nebramycin complex were isolated. The strains proved to be unstable by the antibiotic production property and after three subcultures lost the differences in the complex component composition.