Literature DB >> 2285152

Assay of aspartylglycosylaminase by high-performance liquid chromatography.

V Kaartinen1, I Mononen.   

Abstract

An aspartylglycosylaminase assay based on high-performance liquid chromatographic analysis of the substrate aspartylglucosamine and product aspartate is described. Aspartylglucosamine and aspartate are derivatized with phenylisothiocyanate and resolved by reverse-phase chromatography. The detection limit for the compounds is 2 pmol. The method can be used for analysis of aspartylglycosylaminase activity in crude cell extracts and tissue samples.

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Year:  1990        PMID： 2285152     DOI： 10.1016/0003-2697(90)90140-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Aspartylglycosaminuria in the Finnish population: identification of two point mutations in the heavy chain of glycoasparaginase.

Authors:  I Mononen; N Heisterkamp; V Kaartinen; J C Williams; J R Yates; P R Griffin; L E Hood; J Groffen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

2.  Early initiation of enzyme replacement therapy improves metabolic correction in the brain tissue of aspartylglycosaminuria mice.

Authors:  Ulla Dunder; Pirjo Valtonen; Eira Kelo; Ilkka Mononen
Journal:  J Inherit Metab Dis       Date:  2010-07-06       Impact factor: 4.982

3.  High prevalence of aspartylglycosaminuria among school-age children in eastern Finland.

Authors:  T Mononen; I Mononen; R Matilainen; E Airaksinen
Journal:  Hum Genet       Date:  1991-07       Impact factor: 4.132

Review 4.  Aspartylglycosaminuria: a review.

Authors:  Maria Arvio; Ilkka Mononen
Journal:  Orphanet J Rare Dis       Date:  2016-12-01       Impact factor: 4.123
  4 in total

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