Literature DB >> 2285145

Removal of artifactual bands associated with the presence of 2-mercaptoethanol in two-dimensional polyacrylamide gel electrophoresis.

A Beis1, A Lazou.   

Abstract

A method for eliminating artifactual bands due to the presence of 2-mercaptoethanol in two-dimensional gels is described. The method is based on a modification of the procedure of application of the first dimension gel to the SDS slab gel. 2-Mercaptoethanol is removed during equilibration and replaced by iodoacetamide. The use of iodoacetamide improves the recovery of proteins and results in a better detection of them.

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Year:  1990        PMID： 2285145     DOI： 10.1016/0003-2697(90)90132-s

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  4 in total

1.  Purification of xyloglucan endotransglycosylases (XETs): a generally applicable and simple method based on reversible formation of an enzyme-substrate complex.

Authors:  N M Steele; S C Fry
Journal:  Biochem J       Date:  1999-05-15       Impact factor: 3.857

2.  Changing complexity of endogenous lectin activities during juvenile development of Xenopus laevis.

Authors:  N C Milos; Y N Frunchak
Journal:  Rouxs Arch Dev Biol       Date:  1993-01

3.  Annexins in Paramecium cells. Involvement in site-specific positioning of secretory organelles.

Authors:  M Knochel; R Kissmehl; J D Wissmann; M Momayezi; J Hentschel; H Plattner; R D Burgoyne
Journal:  Histochem Cell Biol       Date:  1996-04       Impact factor: 4.304

4.  Zinc rapidly induces a metal response element-binding factor.

Authors:  M Czupryn; W E Brown; B L Vallee
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205
  4 in total

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