Literature DB >> 22851288

An unexpected promiscuous activity of 4-oxalocrotonate tautomerase: the cis-trans isomerisation of nitrostyrene.

Ellen Zandvoort1, Edzard M Geertsema, Bert-Jan Baas, Wim J Quax, Gerrit J Poelarends.   

Abstract

Serendipitous switch: While exploring cis-nitrostyrene as a potential electrophile in Michael-type addition reactions catalysed by the enzyme 4-oxalocrotonate tautomerase (4-OT), it was unexpectedly found that 4-OT catalyses the isomerisation of cis-nitrostyrene to trans-nitrostyrene (k(cat) /K(m) = 1.9×10(3)  M(-1)  s(-1) ).
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 22851288     DOI： 10.1002/cbic.201200371

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  3 in total

1.  Engineering a Promiscuous Tautomerase into a More Efficient Aldolase for Self-Condensations of Linear Aliphatic Aldehydes.

Authors:  Mehran Rahimi; Jan-Ytzen van der Meer; Edzard M Geertsema; Gerrit J Poelarends
Journal:  Chembiochem       Date:  2017-05-30       Impact factor: 3.164

2.  Understanding the different reactivity of (Z)- and (E)-β-nitrostyrenes in [3+2] cycloaddition reactions. An MEDT study.

Authors:  Mar Ríos-Gutiérrez; Luis R Domingo; Radomir Jasiński
Journal:  RSC Adv       Date:  2021-03-04       Impact factor: 3.361

3.  Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidase.

Authors:  Bert-Jan Baas; Ellen Zandvoort; Anna A Wasiel; Gerrit J Poelarends
Journal:  FEBS Open Bio       Date:  2014-07-09       Impact factor: 2.693
  3 in total

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