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Literature DB >> 22848278

Elevated expression of solute carrier family 22 member 18 increases the sensitivity of U251 glioma cells to BCNU.

Sheng-Hua Chu¹, Yan-Bin Ma, Dong-Fu Feng, Hong Zhang, Jian-Hua Qiu, Zhi-An Zhu.

Abstract

Previous studies showed that solute carrier family 22 member 18 (SLC22A18) is involved in tumorigenesis. The aim of this study was to examine the role of SLC22A18 in glioma cells. Glioma U251 cells were transfected with the human SLC22A18 gene. Transfection of the empty vector pcDNA3.1 was used as a negative control. Sensitivity to BCNU was measured by Annexin V staining. The expression of caspase-3 and bcl-2 was determined by immunohistochemistry. The transfection was confirmed by PCR, RT-PCR and Western blotting. Augmented apoptotic cell death was observed in the SLC22A18-transfected cells, compared to the non-transfected cells or cells with the empty vector. Caspase-3 expression increased in U251-SLC22A18 cells, whereas the bcl-2 expression decreased. These results indicated that SLC22A18 has a pro-apoptotic function in glioma cells.

Entities: Chemical Disease Gene Species

Year: 2011 PMID： 22848278 PMCID： PMC3406540 DOI： 10.3892/ol.2011.371

Source DB: PubMed Journal: Oncol Lett ISSN： 1792-1074 Impact factor: 2.967

8 in total

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Authors: Steffen Albrecht; Wolfgang Hartmann; Farzad Houshdaran; Arend Koch; Barbara Gärtner; Dirk Prawitt; Bernhard U Zabel; Pierre Russo; Dietrich Von Schweinitz; Torsten Pietsch
Journal: Int J Cancer Date: 2004-09-10 Impact factor: 7.396

2. Gain of imprinting of SLC22A18 sense and antisense transcripts in human breast cancer.

Authors: E Gallagher; A Mc Goldrick; W Y Chung; O Mc Cormack; M Harrison; M Kerin; P A Dervan; A Mc Cann
Journal: Genomics Date: 2006-04-19 Impact factor: 5.736

3. C-Met antisense oligodeoxynucleotide inhibits growth of glioma cells.

Authors: Shenghua Chu; Xianhou Yuan; Zhiqiang Li; Pucha Jiang; Jie Zhang
Journal: Surg Neurol Date: 2006-06

4. Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by interacting with Dnmt1.

Authors: Faizaan Mohammad; Tanmoy Mondal; Natalia Guseva; Gaurav Kumar Pandey; Chandrasekhar Kanduri
Journal: Development Date: 2010-06-23 Impact factor: 6.868

5. Tumor suppressor candidate TSSC5 is regulated by UbcH6 and a novel ubiquitin ligase RING105.

Authors: H Y Yamada; G J Gorbsky
Journal: Oncogene Date: 2006-03-02 Impact factor: 9.867

6. Somatic mutation of TSSC5, a novel imprinted gene from human chromosome 11p15.5.

Authors: M P Lee; C Reeves; A Schmitt; K Su; T D Connors; R J Hu; S Brandenburg; M J Lee; G Miller; A P Feinberg
Journal: Cancer Res Date: 1998-09-15 Impact factor: 12.701

7. Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG44 to BCNU.

Authors: Youxin Zhou; Wenshuai Li; Qinian Xu; Yulun Huang
Journal: J Exp Clin Cancer Res Date: 2010-10-04

8. Transcriptional map of 170-kb region at chromosome 11p15.5: identification and mutational analysis of the BWR1A gene reveals the presence of mutations in tumor samples.

Authors: C Schwienbacher; S Sabbioni; M Campi; A Veronese; G Bernardi; A Menegatti; I Hatada; T Mukai; H Ohashi; G Barbanti-Brodano; C M Croce; M Negrini
Journal: Proc Natl Acad Sci U S A Date: 1998-03-31 Impact factor: 11.205

8 in total

6 in total

Elevated expression of solute carrier family 22 member 18 increases the sensitivity of U251 glioma cells to BCNU.

1. Allelic loss but absence of mutations in the polyspecific transporter gene BWR1A on 11p15.5 in hepatoblastoma.

2. Gain of imprinting of SLC22A18 sense and antisense transcripts in human breast cancer.

3. C-Met antisense oligodeoxynucleotide inhibits growth of glioma cells.

4. Kcnq1ot1 noncoding RNA mediates transcriptional gene silencing by interacting with Dnmt1.

5. Tumor suppressor candidate TSSC5 is regulated by UbcH6 and a novel ubiquitin ligase RING105.

6. Somatic mutation of TSSC5, a novel imprinted gene from human chromosome 11p15.5.

7. Elevated expression of Dickkopf-1 increases the sensitivity of human glioma cell line SHG44 to BCNU.

8. Transcriptional map of 170-kb region at chromosome 11p15.5: identification and mutational analysis of the BWR1A gene reveals the presence of mutations in tumor samples.

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4. Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo.

5. Upregulation of SATB1 is associated with the development and progression of glioma.

6. Predictive value of the SLC22A18 protein expression in glioblastoma patients receiving temozolomide therapy.