Alison S F Elder1, Andrew D Bersten2, Gino T P Saccone3, Dani-Louise Dixon2. 1. Department of Critical Care Medicine, Flinders University, Adelaide, SA, Australia. Electronic address: a.elder@flinders.edu.au. 2. Department of Critical Care Medicine, Flinders University, Adelaide, SA, Australia. 3. Department of Surgery, Flinders University, Adelaide, SA, Australia.
Abstract
BACKGROUND: The synthetic tripeptide feG (D-Phe-D-Glu-Gly) is a novel pharmacologic agent that decreases neutrophil recruitment, infiltration, and activation in various animal models of inflammatory disease. We aimed to investigate the effect of feG as both a preventive treatment when administered before acute lung injury and as a therapeutic treatment administered following initiation of acute lung injury. METHODS: Lung injury was assessed following prophylactic or therapeutic intratracheal feG administration in a “two-hit” rodent model of acute pancreatitis plus intratracheal lipopolysaccharide. RESULTS: Following both prophylactic and therapeutic feG administration, there were significant improvements in arterial blood oxygenation and respiratory mechanics and decreased lung edema, BAL protein concentration, histologic tissue injury scores, BAL cell infiltration, and lung myeloperoxidase activity. Most indices of lung damage were reduced to baseline control values. CONCLUSIONS: feG reduced leukocyte infiltration, ameliorated the severity of inflammatory damage, and restored lung function when administered either prophylactically or therapeutically in a two-hit rat model of acute pancreatitis plus intratracheal lipopolysaccharide.
BACKGROUND: The synthetic tripeptidefeG (D-Phe-D-Glu-Gly) is a novel pharmacologic agent that decreases neutrophil recruitment, infiltration, and activation in various animal models of inflammatory disease. We aimed to investigate the effect of feG as both a preventive treatment when administered before acute lung injury and as a therapeutic treatment administered following initiation of acute lung injury. METHODS:Lung injury was assessed following prophylactic or therapeutic intratracheal feG administration in a “two-hit” rodent model of acute pancreatitis plus intratracheallipopolysaccharide. RESULTS: Following both prophylactic and therapeutic feG administration, there were significant improvements in arterial blood oxygenation and respiratory mechanics and decreased lung edema, BAL protein concentration, histologic tissue injury scores, BAL cell infiltration, and lung myeloperoxidase activity. Most indices of lung damage were reduced to baseline control values. CONCLUSIONS:feG reduced leukocyte infiltration, ameliorated the severity of inflammatory damage, and restored lung function when administered either prophylactically or therapeutically in a two-hit rat model of acute pancreatitis plus intratracheallipopolysaccharide.
Authors: Chris D St Laurent; Katherine E St Laurent; Ron D Mathison; A Dean Befus Journal: Am J Physiol Regul Integr Comp Physiol Date: 2015-01-28 Impact factor: 3.619
Authors: Alison S F Elder; Andrew D Bersten; Gino T P Saccone; Claudine S Bonder; Dani-Louise Dixon Journal: Lung Date: 2019-07-12 Impact factor: 2.584
Authors: Shailesh Bihari; Dani-Louise Dixon; Mark D Lawrence; Dylan De Bellis; Claudine S Bonder; David P Dimasi; Andrew D Bersten Journal: Pflugers Arch Date: 2017-04-29 Impact factor: 3.657