Yanlin Li1, Zhaofang Zeng, Shaoli Deng. 1. Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, China.
Abstract
OBJECTIVE: To study the human migration inhibitory factor (MIF) level in tuberculosis (TB) patients, and the relationship between MIF-794CATT microsatellite polymorphism and susceptibility of TB in Southwest China. METHODS: TB patients (n=151) and healthy unrelated controls (n=149) were recruited for this study. Genomic DNA was extracted, and then amplified by polymerase chain reaction (PCR). MIF-794CATT(5-8) microsatellite polymorphism was genotyped by DNA sequencing. MIF level was detected by ELISA. RESULTS: In the TB group, the repeat number of 7/7 and 7/8 (17.89%) was significantly higher than that of the control group (8.05%), and the serum MIF level was also much higher than that of the healthy controls (705.21 ± 67.98 vs. 355.31 ± 57.29 pg/mL, p<0.01). CONCLUSION: The appearance of MIF-794CATT 7/7 and 7/8 is associated with susceptibility to TB, and may play an important role in the occurrence and development of TB in Southwest China.
OBJECTIVE: To study the humanmigration inhibitory factor (MIF) level in tuberculosis (TB) patients, and the relationship between MIF-794CATT microsatellite polymorphism and susceptibility of TB in Southwest China. METHODS: TB patients (n=151) and healthy unrelated controls (n=149) were recruited for this study. Genomic DNA was extracted, and then amplified by polymerase chain reaction (PCR). MIF-794CATT(5-8) microsatellite polymorphism was genotyped by DNA sequencing. MIF level was detected by ELISA. RESULTS: In the TB group, the repeat number of 7/7 and 7/8 (17.89%) was significantly higher than that of the control group (8.05%), and the serum MIF level was also much higher than that of the healthy controls (705.21 ± 67.98 vs. 355.31 ± 57.29 pg/mL, p<0.01). CONCLUSION: The appearance of MIF-794CATT 7/7 and 7/8 is associated with susceptibility to TB, and may play an important role in the occurrence and development of TB in Southwest China.
Authors: Duncan Reid; Sheela Shenoi; Ravesh Singh; Max Wang; Vinod Patel; Rituparna Das; Keshni Hiramen; Yunus Moosa; Francois Eksteen; Anthony P Moll; Thumbi Ndung'u; Victoria Kasprowicz; Lin Leng; Gerald H Friedland; Richard Bucala Journal: Cytokine X Date: 2019-02-16
Authors: Jun Wang; Bin Sheng; XiaoPeng Li; JianMin Sun; Lin Shi; WenTao Wei; GuoWei Wang; XueJun Cao Journal: Medicine (Baltimore) Date: 2020-07-24 Impact factor: 1.817