Literature DB >> 22842454

High resolution melting analysis for detection of variable number of tandem repeats polymorphism of XRCC5.

Mehrdad Rajaei1, Iraj Saadat, Mostafa Saadat.   

Abstract

Several polymorphisms in the XRCC5 (X-ray repair cross-complementing 5; OMIM: 194364) were reported. Polymorphism of variable number of tandem repeats (VNTR) in the promoter region of XRCC5 (rs6147172) was reported. The main aim of the present study is to introduce the high resolution melting analysis (HRMA) method for genotyping of the polymorphism of XRCC5 VNTR. Genotypes of XRCC5 VNTR were determined by HRMA and conventional PCR method, and confirmed by DNA sequencing. The results for genotyping using HRMA and conventional PCR showed 100% concordance. All genotypes of the XRCC5 VNTR polymorphism could be accurately detected by HRMA.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 22842454     DOI： 10.1016/j.bbrc.2012.07.109

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  3 in total

1.  Association between polymorphisms at promoters of XRCC5 and XRCC6 genes and risk of breast cancer.

Authors:  Mehrdad Rajaei; Iraj Saadat; Shahpour Omidvari; Mostafa Saadat
Journal:  Med Oncol       Date:  2014-03-11       Impact factor: 3.064

2.  Susceptibility to gastric cancer and polymorphisms of insertion/deletion at the intron 3 of the XRCC4 and VNTR at the promoter region of the XRCC5.

Authors:  Mostafa Saadat; Samira Pashaei; Foroozan Amerizade
Journal:  Pathol Oncol Res       Date:  2014-12-20       Impact factor: 3.201

3.  Association of XRCC5 VNTR polymorphism with the development of chronic myeloid leukemia.

Authors:  Manjula Gorre; Prajitha Edathara Mohandas; Sailaja Kagita; Sandhya Annamaneni; Raghunadharao Digumarti; Vishnupriya Satti
Journal:  Tumour Biol       Date:  2013-08-28
  3 in total

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