Vandana Rai1, Upendra Yadav, Pradeep Kumar, Sushil Kumar Yadav. 1. Human Molecular Genetics Laboratory, Department of Biotechnology, VBS Purvanchal University, Jaunpur - 222 001, Uttar Pradesh, India. raivandana@rediffmail.com
Abstract
OBJECTIVE: The aim of the present study was to investigate the distribution of methylenetetrahydrofolate reductase (C677T) polymorphism in the Muslim population of eastern Uttar Pradesh. MATERIALS AND METHODS: Total 56 subjects were analysed for MTHFR C677T polymorphism. C677T mutation analysis was done according to the PCR-RFLP (Polymerase chain reaction-Restriction fragment length polymorphism) method. RESULTS: The frequencies of three genotypes CC, CT, and TT were 0.857, 0.125, and 0.07, respectively, and the frequency of mutated T allele was found to be 0.080. CONCLUSION: Genotypes and allele frequencies revealed the low prevalence of MTHFR C677T polymorphism in Indin Muslims. C677T mutation has been suggested to be positively associated with the risk of several congenital and multifactorial disorders. The low frequency of T/T genotype in the Muslim population may be due to malnutrition in pregnant women, because of insufficient intake of folate is considered to be a survival disadvantage for foetuses with T/T genotype.
OBJECTIVE: The aim of the present study was to investigate the distribution of methylenetetrahydrofolate reductase (C677T) polymorphism in the Muslim population of eastern Uttar Pradesh. MATERIALS AND METHODS: Total 56 subjects were analysed for MTHFR C677T polymorphism. C677T mutation analysis was done according to the PCR-RFLP (Polymerase chain reaction-Restriction fragment length polymorphism) method. RESULTS: The frequencies of three genotypes CC, CT, and TT were 0.857, 0.125, and 0.07, respectively, and the frequency of mutated T allele was found to be 0.080. CONCLUSION: Genotypes and allele frequencies revealed the low prevalence of MTHFR C677T polymorphism in Indin Muslims. C677T mutation has been suggested to be positively associated with the risk of several congenital and multifactorial disorders. The low frequency of T/T genotype in the Muslim population may be due to malnutrition in pregnant women, because of insufficient intake of folate is considered to be a survival disadvantage for foetuses with T/T genotype.