| Literature DB >> 22841738 |
Shilpi Sharma1, Ravikumar Mehta, Rashi Gupta, Michael Schloter.
Abstract
An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.Entities:
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Year: 2012 PMID: 22841738 DOI: 10.1016/j.mimet.2012.07.016
Source DB: PubMed Journal: J Microbiol Methods ISSN: 0167-7012 Impact factor: 2.363