Literature DB >> 22841738

Improved protocol for the extraction of bacterial mRNA from soils.

Shilpi Sharma1, Ravikumar Mehta, Rashi Gupta, Michael Schloter.   

Abstract

An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID: 22841738     DOI: 10.1016/j.mimet.2012.07.016

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  5 in total

1.  Ecotoxicological assessment of pesticides and their combination on rhizospheric microbial community structure and function of Vigna radiata.

Authors:  Varsha Ashok Walvekar; Swati Bajaj; Dileep K Singh; Shilpi Sharma
Journal:  Environ Sci Pollut Res Int       Date:  2017-06-06       Impact factor: 4.223

2.  Nontarget effects of chemical pesticides and biological pesticide on rhizospheric microbial community structure and function in Vigna radiata.

Authors:  Sunil Singh; Rashi Gupta; Madhu Kumari; Shilpi Sharma
Journal:  Environ Sci Pollut Res Int       Date:  2015-03-24       Impact factor: 4.223

3.  Effect of substitution of chemical fertilizer by bioinoculants on plant performance and rhizospheric bacterial community: case study with Cajanus cajan.

Authors:  Richa Sharma; Vijay Laxmi Shrivas; Shilpi Sharma
Journal:  Braz J Microbiol       Date:  2021-01-07       Impact factor: 2.476

4.  Differential ratio amplicons (Ramp ) for the evaluation of RNA integrity extracted from complex environmental samples.

Authors:  Fabien Cholet; Umer Z Ijaz; Cindy J Smith
Journal:  Environ Microbiol       Date:  2019-02-12       Impact factor: 5.491

5.  Effect of Agricultural Amendments on Cajanus cajan (Pigeon Pea) and Its Rhizospheric Microbial Communities--A Comparison between Chemical Fertilizers and Bioinoculants.

Authors:  Rashi Gupta; V S Bisaria; Shilpi Sharma
Journal:  PLoS One       Date:  2015-07-31       Impact factor: 3.240

  5 in total

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