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Literature DB >> 22837324

Modeling uncertainty in single-copy assays for HIV.

Rutao Luo¹, Michael J Piovoso, Ryan Zurakowski.

Abstract

We present a simple computational model of measurement accuracy for single-copy sensitivity assays (SCA) of HIV RNA that was developed from first principles. The model shows that the SCA is significantly right-skewed. Measured virus concentrations of 1 and 10 virions/ml had overlapping 95% confidence intervals and were statistically indistinguishable.

Entities: Disease

Mesh：

Year: 2012 PMID： 22837324 PMCID： PMC3457444 DOI： 10.1128/JCM.01254-12

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

6 in total

1. A Bayesian approach to parameter estimation in HIV dynamical models.

Authors: H Putter; S H Heisterkamp; J M A Lange; F de Wolf
Journal: Stat Med Date: 2002-08-15 Impact factor: 2.373

Review 2. New tools for quantifying HIV-1 reservoirs: plasma RNA single copy assays and beyond.

Authors: Benedict B Hilldorfer; Anthony R Cillo; Guillaume J Besson; Margaret Anne Bedison; John W Mellors
Journal: Curr HIV/AIDS Rep Date: 2012-03 Impact factor: 5.071

3. Multicenter performance evaluation of a new TaqMan PCR assay for monitoring human immunodeficiency virus RNA load.

Authors: L Perrin; J M Pawlotsky; M Bouvier-Alias; C Sarrazin; S Zeuzem; G Colucci
Journal: J Clin Microbiol Date: 2006-09-27 Impact factor: 5.948

4. New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma.

Authors: Sarah Palmer; Ann P Wiegand; Frank Maldarelli; Holly Bazmi; JoAnn M Mican; Michael Polis; Robin L Dewar; Angeline Planta; Shuying Liu; Julia A Metcalf; John W Mellors; John M Coffin
Journal: J Clin Microbiol Date: 2003-10 Impact factor: 5.948

5. HIV model parameter estimates from interruption trial data including drug efficacy and reservoir dynamics.

Authors: Rutao Luo; Michael J Piovoso; Javier Martinez-Picado; Ryan Zurakowski
Journal: PLoS One Date: 2012-07-16 Impact factor: 3.240

6. Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.

Authors: Martin Bengtsson; Martin Hemberg; Patrik Rorsman; Anders Ståhlberg
Journal: BMC Mol Biol Date: 2008-07-17 Impact factor: 2.946

6 in total

1. Implications of Measurement Assay Type in Design of HIV Experiments.

Authors: LaMont Cannon; Aditya Jagarapu; Cesar A Vargas-Garcia; Michael J Piovoso; Ryan Zurakowski
Journal: Proc IEEE Conf Decis Control Date: 2018-01-23

2. Long-term antiretroviral treatment initiated at primary HIV-1 infection affects the size, composition, and decay kinetics of the reservoir of HIV-1-infected CD4 T cells.

Authors: Maria J Buzon; Enrique Martin-Gayo; Florencia Pereyra; Zhengyu Ouyang; Hong Sun; Jonathan Z Li; Michael Piovoso; Amy Shaw; Judith Dalmau; Nadine Zangger; Javier Martinez-Picado; Ryan Zurakowski; Xu G Yu; Amalio Telenti; Bruce D Walker; Eric S Rosenberg; Mathias Lichterfeld
Journal: J Virol Date: 2014-06-25 Impact factor: 5.103

Modeling uncertainty in single-copy assays for HIV.

1. A Bayesian approach to parameter estimation in HIV dynamical models.

Review 2. New tools for quantifying HIV-1 reservoirs: plasma RNA single copy assays and beyond.

3. Multicenter performance evaluation of a new TaqMan PCR assay for monitoring human immunodeficiency virus RNA load.

4. New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma.

5. HIV model parameter estimates from interruption trial data including drug efficacy and reservoir dynamics.

6. Quantification of mRNA in single cells and modelling of RT-qPCR induced noise.

1. Implications of Measurement Assay Type in Design of HIV Experiments.

2. Long-term antiretroviral treatment initiated at primary HIV-1 infection affects the size, composition, and decay kinetics of the reservoir of HIV-1-infected CD4 T cells.

3. Experiment Design for Early Molecular Events in HIV Infection.

4. Modelling HIV-1 2-LTR dynamics following raltegravir intensification.

5. HIV 2-LTR experiment design optimization.

6. Modeling HIV-1 Within-Host Dynamics After Passive Infusion of the Broadly Neutralizing Antibody VRC01.