OBJECTIVE: To evaluate correlations between metabolic activity and implantation potential of transferred embryos in a study based on oxygen (O(2)) consumption (OC) measurements, because O(2) uptake is directly related to the capacity of an embryo to produce energy via adenosine triphosphate. DESIGN: Retrospective cohort study. SETTING: Infertility institute. PATIENT(S): Five hundred seventy-five injected oocytes in 56 first oocyte donation cycles with embryo transfer on day 3. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): We analyzed embryo destination viability and implantation depending on the embryo OC rate obtained from 47,741 measurements (up to 85 measurements per embryo, 2-3 measurements per hour). OC patterns were analyzed in relation to the time elapsed from sperm microinjection, to the final destination of the embryos (transferred, frozen, or discarded), to ongoing pregnancy, and by successful implantation. RESULT(S): OC was found to decrease during embryonic development. OC patterns from 52 hours onward showed the strongest correlation with implantation success. Regarding embryo destination, the same patterns were observed. CONCLUSION(S): OC from individual embryos revealed significant differences, mainly close to the time of transfer, when OC pattern was associated with successful implantation. Therefore, measuring the OC pattern of human embryos culture up to 72 hours could be used to select the embryo with best developmental potential.
OBJECTIVE: To evaluate correlations between metabolic activity and implantation potential of transferred embryos in a study based on oxygen (O(2)) consumption (OC) measurements, because O(2) uptake is directly related to the capacity of an embryo to produce energy via adenosine triphosphate. DESIGN: Retrospective cohort study. SETTING: Infertility institute. PATIENT(S): Five hundred seventy-five injected oocytes in 56 first oocyte donation cycles with embryo transfer on day 3. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): We analyzed embryo destination viability and implantation depending on the embryo OC rate obtained from 47,741 measurements (up to 85 measurements per embryo, 2-3 measurements per hour). OC patterns were analyzed in relation to the time elapsed from sperm microinjection, to the final destination of the embryos (transferred, frozen, or discarded), to ongoing pregnancy, and by successful implantation. RESULT(S): OC was found to decrease during embryonic development. OC patterns from 52 hours onward showed the strongest correlation with implantation success. Regarding embryo destination, the same patterns were observed. CONCLUSION(S): OC from individual embryos revealed significant differences, mainly close to the time of transfer, when OC pattern was associated with successful implantation. Therefore, measuring the OC pattern of human embryos culture up to 72 hours could be used to select the embryo with best developmental potential.
Authors: Corie M Owen; Melissa A Johnson; Katherine A Rhodes-Long; Diana J Gumber; Moises Barceló-Fimbres; Joy L Altermatt; Lino Fernando Campos-Chillon Journal: J Anim Sci Date: 2022-03-01 Impact factor: 3.159
Authors: Sharon Anderson; Peining Xu; Alexander J Frey; Jason R Goodspeed; Mary T Doan; John J Orris; Nicolle Clements; Michael J Glassner; Nathaniel W Snyder Journal: Syst Biol Reprod Med Date: 2021-12-28 Impact factor: 3.061
Authors: Giovana D Catandi; Yusra M Obeidat; Corey D Broeckling; Thomas W Chen; Adam J Chicco; Elaine M Carnevale Journal: Reproduction Date: 2021-04 Impact factor: 3.906
Authors: José C Rocha; Felipe Passalia; Felipe D Matos; Marc P Maserati; Mayra F Alves; Tamie G de Almeida; Bruna L Cardoso; Andrea C Basso; Marcelo F G Nogueira Journal: JBRA Assist Reprod Date: 2016-08-01
Authors: Maria José de Los Santos; Pilar Gámiz; José María de Los Santos; Josep Lluís Romero; Nicolás Prados; Cristina Alonso; José Remohí; Francisco Dominguez Journal: PLoS One Date: 2015-11-12 Impact factor: 3.240