OBJECTIVE: To reevaluate the efficiency of the 12 differentially methylated regions (DMRs) used in the methylated DNA immunoprecipitation (MeDIP) real-time quantitative polymerase chain reaction (real-time qPCR) based approach, develop an improved version of the diagnostic formula and perform a larger validation study. METHODS: Twelve selected DMRs were checked for copy number variants in the Database of Genomic Variants. The DMRs located within copy number variants were excluded from the analysis. One hundred and seventy-five maternal peripheral blood samples were used to reconstruct and evaluate the new diagnostic formula and for a larger-scale blinded validation study using MeDIP real-time qPCR. RESULTS: Seven DMRs entered the final model of the prediction equation and a larger blinded validation study demonstrated 100% sensitivity and 99.2% specificity. No significant evidence for association was observed between cell free fetal DNA concentration and D value. CONCLUSION: The MeDIP real-time qPCR method for noninvasive prenatal diagnosis of trisomy 21 was confirmed and revalidated in 175 samples with satisfactory results demonstrating that it is accurate and reproducible. We are currently working towards simplification of the method to make it more robust and therefore easily, accurately, and rapidly reproduced and adopted by other laboratories. Nevertheless, larger scale validation studies are necessary before the MeDIP real-time qPCR-based method could be applied in clinical practice.
OBJECTIVE: To reevaluate the efficiency of the 12 differentially methylated regions (DMRs) used in the methylated DNA immunoprecipitation (MeDIP) real-time quantitative polymerase chain reaction (real-time qPCR) based approach, develop an improved version of the diagnostic formula and perform a larger validation study. METHODS: Twelve selected DMRs were checked for copy number variants in the Database of Genomic Variants. The DMRs located within copy number variants were excluded from the analysis. One hundred and seventy-five maternal peripheral blood samples were used to reconstruct and evaluate the new diagnostic formula and for a larger-scale blinded validation study using MeDIP real-time qPCR. RESULTS: Seven DMRs entered the final model of the prediction equation and a larger blinded validation study demonstrated 100% sensitivity and 99.2% specificity. No significant evidence for association was observed between cell free fetal DNA concentration and D value. CONCLUSION: The MeDIP real-time qPCR method for noninvasive prenatal diagnosis of trisomy 21 was confirmed and revalidated in 175 samples with satisfactory results demonstrating that it is accurate and reproducible. We are currently working towards simplification of the method to make it more robust and therefore easily, accurately, and rapidly reproduced and adopted by other laboratories. Nevertheless, larger scale validation studies are necessary before the MeDIP real-time qPCR-based method could be applied in clinical practice.
Authors: Ji Hyae Lim; Da Eun Lee; So Yeon Park; Do Jin Kim; Hyun Kyong Ahn; You Jung Han; Moon Young Kim; Hyun Mee Ryu Journal: BMC Med Genomics Date: 2014-01-08 Impact factor: 3.063
Authors: Marios Ioannides; Elisavet A Papageorgiou; Anna Keravnou; Evdokia Tsaliki; Christiana Spyrou; Michael Hadjidaniel; Carolina Sismani; George Koumbaris; Philippos C Patsalis Journal: Mol Cytogenet Date: 2014-11-01 Impact factor: 2.009
Authors: Jacob Mørup Schlütter; Ida Kirkegaard; Olav Bjørn Petersen; Nanna Larsen; Britta Christensen; David M Hougaard; Steen Kølvraa; Niels Uldbjerg Journal: PLoS One Date: 2014-09-04 Impact factor: 3.240
Authors: Elisavet A Papageorgiou; George Koumbaris; Elena Kypri; Michael Hadjidaniel; Philippos C Patsalis Journal: Genes (Basel) Date: 2014-04-09 Impact factor: 4.096