Literature DB >> 2283027

Cloning, mapping and gene product identification of rhaT from Escherichia coli K12.

L Baldomá1, J Badía, G Sweet, J Aguilar.   

Abstract

Rhamnose utilization requires the function of a specific rhamnose transport system. Rhamnose transport mutants have been isolated and characterized. The structural gene, rhaT, encoding the rhamnose permease has been cloned from Escherichia coli. rhaT has been mapped in the rha locus (87.7 min) by analysis of cotransduction with glpK and other rha markers. The precise location of the gene has been determined by complementation analysis of rhamnose transport mutants transformed with recombinant plasmids containing different fragments of the cloned region. Gene order (counterclockwise) is established as glpK . . . rhaT-rhaR-rhaS-rhaB-rhaA-rhaD. The gene product has been identified by expression of rhaT in a T7 RNA polymerase/promoter system. This 23 kDa protein has been assigned to the rhaT product and has been shown to be located in the cell membrane.

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Year:  1990        PMID： 2283027     DOI： 10.1016/0378-1097(90)90353-r

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

Review 1.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

2.  Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.

Authors:  G Plunkett; V Burland; D L Daniels; F R Blattner
Journal:  Nucleic Acids Res       Date:  1993-07-25       Impact factor: 16.971

3.  Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon.

Authors:  A Haldimann; L L Daniels; B L Wanner
Journal:  J Bacteriol       Date:  1998-03       Impact factor: 3.490

4.  Cyclic AMP receptor protein and RhaR synergistically activate transcription from the L-rhamnose-responsive rhaSR promoter in Escherichia coli.

Authors:  Jason R Wickstrum; Thomas J Santangelo; Susan M Egan
Journal:  J Bacteriol       Date:  2005-10       Impact factor: 3.490

Review 5.  Functions of the gene products of Escherichia coli.

Authors:  M Riley
Journal:  Microbiol Rev       Date:  1993-12

6.  Comparative genomics and functional analysis of rhamnose catabolic pathways and regulons in bacteria.

Authors:  Irina A Rodionova; Xiaoqing Li; Vera Thiel; Sergey Stolyar; Krista Stanton; James K Fredrickson; Donald A Bryant; Andrei L Osterman; Aaron A Best; Dmitry A Rodionov
Journal:  Front Microbiol       Date:  2013-12-23       Impact factor: 5.640

7.  The l-rhamnose-dependent regulator RhaS and its target promoters from Escherichia coli expand the genetic toolkit for regulatable gene expression in the acetic acid bacterium Gluconobacter oxydans.

Authors:  Philipp Moritz Fricke; Mandy Lynn Gries; Maurice Mürköster; Marvin Höninger; Jochem Gätgens; Michael Bott; Tino Polen
Journal:  Front Microbiol       Date:  2022-08-16       Impact factor: 6.064
  7 in total

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