Literature DB >> 2283000

Two different mRNAs coding for identical elongation factor 1 alpha (EF-1 alpha) polypeptides in Xenopus laevis embryos.

A Pöting1, K Danker, L Hartmann, M Köster, D Wedlich, W Knöchel.   

Abstract

Two related but clearly different cDNA clones corresponding to elongation factor 1 alpha (EF-1 alpha) mRNAs were isolated from a Xenopus laevis gastrula-stage library. Whereas the nucleotide sequences of these two cDNAs differ within the coding region at 49 out of 1386 positions (3.5%), the derived amino acid sequences are completely identical, thereby indicating a substantial evolutionary constraint on this translation factor. Southern-blot analysis of genomic DNA suggests that, besides the two closely related EF-1 alpha genes investigated in this study, other more-distantly related genes may exist in the X. laevis genome. Transcription of EF-1 alpha genes during oogenesis and embryonic development was studied by Northern-blot analysis and by in situ hybridizations. A high amount of EF-1 alpha mRNA was detected in previtellogenic oocytes. At later stages of embryonic development, EF-1 alpha mRNA was found to be accumulated in translationally active tissues.

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Year:  1990        PMID： 2283000     DOI： 10.1111/j.1432-0436.1990.tb00543.x

Source DB:  PubMed          Journal:  Differentiation        ISSN： 0301-4681            Impact factor:   3.880


  5 in total

1.  Nucleotide sequence of rat elongation factor-1 alpha cDNA.

Authors:  T Shirasawa; K Sakamoto; T Akashi; H Takahashi; A Kawashima
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

2.  Transcription patterns of four different fork head/HNF-3 related genes (XFD-4, 6, 9 and 10) in Xenopus laevis embryos.

Authors:  Michaela Scheucher; Petra Dege; Jutta Lef; Sigurd Hille; Walter Knöchel
Journal:  Rouxs Arch Dev Biol       Date:  1995-01

3.  Gene structure and alternative splicing of XFG 5-1, a X. laevis Zn finger protein with RNA homopolymer binding activity.

Authors:  M Köster; S Hille; T Pieler; W Knöchel
Journal:  Mol Biol Rep       Date:  1993-10       Impact factor: 2.316

4.  Germ cell-specific expression of a gene encoding eukaryotic translation elongation factor 1 alpha (eEF-1 alpha) and generation of eEF-1 alpha retropseudogenes in Xenopus laevis.

Authors:  B Abdallah; J Hourdry; P A Krieg; H Denis; A Mazabraud
Journal:  Proc Natl Acad Sci U S A       Date:  1991-10-15       Impact factor: 11.205

5.  Two forms of elongation factor 1 alpha (EF-1 alpha O and 42Sp50), present in oocytes, but absent in somatic cells of Xenopus laevis.

Authors:  S Deschamps; J Morales; A Mazabraud; M le Maire; H Denis; D D Brown
Journal:  J Cell Biol       Date:  1991-09       Impact factor: 10.539
  5 in total

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