BACKGROUND: Lymphatic metastasis is a critical determinant of prognosis in human gastrointestinal cancers. Studies suggest that lymphatic metastasis has been linked to lymphangiogenesis, the growth of lymphatic vessels, while the mechanisms of tumor lymphangiogenesis remain poorly characterized. METHODS: Human gastric cancer cells, MKN45, were implanted under the gastric submucosa of nude mice receiving green fluorescent protein-positive bone marrow (BM) transplants. In addition, MKN45 cells were subcutaneously injected into the back of each mouse as a model of human tumor xenografts. The tumor tissue was analyzed 3 weeks after implantation. RESULTS: The mice with MKN45 cells represent recruitment and incorporation of BM-derived lymphatic endothelial progenitor cells (LEPC) into gastric lymphatics. Moreover, in a xenograft model, MKN45 cells induced lymphangiogenesis as well as recruitment of BM-derived LEPC in tumor lymphatics in a xenograft model. CONCLUSIONS: These findings of this study suggest that human gastric adenocarcinoma induces tumor lymphangiogenesis via recruitment of LEPC from BM.
BACKGROUND: Lymphatic metastasis is a critical determinant of prognosis in humangastrointestinal cancers. Studies suggest that lymphatic metastasis has been linked to lymphangiogenesis, the growth of lymphatic vessels, while the mechanisms of tumor lymphangiogenesis remain poorly characterized. METHODS:Humangastric cancer cells, MKN45, were implanted under the gastric submucosa of nude mice receiving green fluorescent protein-positive bone marrow (BM) transplants. In addition, MKN45 cells were subcutaneously injected into the back of each mouse as a model of humantumor xenografts. The tumor tissue was analyzed 3 weeks after implantation. RESULTS: The mice with MKN45 cells represent recruitment and incorporation of BM-derived lymphatic endothelial progenitor cells (LEPC) into gastric lymphatics. Moreover, in a xenograft model, MKN45 cells induced lymphangiogenesis as well as recruitment of BM-derived LEPC in tumor lymphatics in a xenograft model. CONCLUSIONS: These findings of this study suggest that humangastric adenocarcinoma induces tumor lymphangiogenesis via recruitment of LEPC from BM.
Authors: Dontscho Kerjaschki; Nicole Huttary; Ingrid Raab; Heinz Regele; Katalin Bojarski-Nagy; Gregor Bartel; Stefan M Kröber; Hildegard Greinix; Agathe Rosenmaier; Franz Karlhofer; Nikolaus Wick; Peter R Mazal Journal: Nat Med Date: 2006-01-15 Impact factor: 53.440
Authors: D Lyden; K Hattori; S Dias; C Costa; P Blaikie; L Butros; A Chadburn; B Heissig; W Marks; L Witte; Y Wu; D Hicklin; Z Zhu; N R Hackett; R G Crystal; M A Moore; K A Hajjar; K Manova; R Benezra; S Rafii Journal: Nat Med Date: 2001-11 Impact factor: 53.440
Authors: T Asahara; H Masuda; T Takahashi; C Kalka; C Pastore; M Silver; M Kearne; M Magner; J M Isner Journal: Circ Res Date: 1999-08-06 Impact factor: 17.367
Authors: Lisa Volk-Draper; Radhika Patel; Nihit Bhattarai; Jie Yang; Andrew Wilber; David DeNardo; Sophia Ran Journal: Am J Pathol Date: 2019-08-15 Impact factor: 4.307
Authors: J J Morton; G Bird; S B Keysar; D P Astling; T R Lyons; R T Anderson; M J Glogowska; P Estes; J R Eagles; P N Le; G Gan; B McGettigan; P Fernandez; N Padilla-Just; M Varella-Garcia; J I Song; D W Bowles; P Schedin; A-C Tan; D R Roop; X-J Wang; Y Refaeli; A Jimeno Journal: Oncogene Date: 2015-04-20 Impact factor: 9.867