PURPOSE: T cell activation as well as unresponsiveness has been described in separate studies in sepsis. Our aim was to establish the coexistence of both T cell fate in human sepsis. PATIENTS AND METHODS: This is a cross-sectional study of 48 patients presenting with severe sepsis or septic shock and 15 healthy controls. Cytofluorometric techniques were used to quantify T cell activation, apoptosis, proliferation, expression of costimulatory molecules, and cytokine secretion. RESULTS: Patients with sepsis were characterized by a significant increase in the percentage of activated T cell subsets, as measured using CD69 marker, compared with healthy controls (P<.05). T cell proliferation as measured through Ki67 expression was obvious in infected patients for both CD4 and CD8 T cell subsets compared with controls (P ≤.006). T cell subset apoptosis as measured using Hoechst dye was also increased in infected patients compared with controls (P ≤.002). CD4 T cell proliferation was correlated with interleukin 2 secretion (R(2)=0.84, P<.001), whereas up-regulation of CD4 T cell apoptosis was correlated with CTLA-4 expression (R(2)=0.24, P=.001). No such similar relationship was observed for CD8(+) T cells. CONCLUSIONS: Concomitant T cell proliferation and T cell apoptosis are observed in human sepsis, being related to a different pathway.
PURPOSE: T cell activation as well as unresponsiveness has been described in separate studies in sepsis. Our aim was to establish the coexistence of both T cell fate in humansepsis. PATIENTS AND METHODS: This is a cross-sectional study of 48 patients presenting with severe sepsis or septic shock and 15 healthy controls. Cytofluorometric techniques were used to quantify T cell activation, apoptosis, proliferation, expression of costimulatory molecules, and cytokine secretion. RESULTS:Patients with sepsis were characterized by a significant increase in the percentage of activated T cell subsets, as measured using CD69 marker, compared with healthy controls (P<.05). T cell proliferation as measured through Ki67 expression was obvious in infectedpatients for both CD4 and CD8 T cell subsets compared with controls (P ≤.006). T cell subset apoptosis as measured using Hoechst dye was also increased in infectedpatients compared with controls (P ≤.002). CD4 T cell proliferation was correlated with interleukin 2 secretion (R(2)=0.84, P<.001), whereas up-regulation of CD4 T cell apoptosis was correlated with CTLA-4 expression (R(2)=0.24, P=.001). No such similar relationship was observed for CD8(+) T cells. CONCLUSIONS: Concomitant T cell proliferation and T cell apoptosis are observed in humansepsis, being related to a different pathway.
Authors: Mara A Serbanescu; Kimberly M Ramonell; Annette Hadley; Lindsay M Margoles; Rohit Mittal; John D Lyons; Zhe Liang; Craig M Coopersmith; Mandy L Ford; Kevin W McConnell Journal: J Leukoc Biol Date: 2016-06-10 Impact factor: 4.962
Authors: Javier Cabrera-Perez; Stephanie A Condotta; Vladimir P Badovinac; Thomas S Griffith Journal: J Leukoc Biol Date: 2014-05-02 Impact factor: 4.962
Authors: Anis Chaari; Karim Abdel Hakim; Kamel Bousselmi; Mahmoud Etman; Mohamed El Bahr; Ahmed El Saka; Eman Hamza; Mohamed Ismail; Elsayed Mahmoud Khalil; Vipin Kauts; William Francis Casey Journal: World J Gastrointest Oncol Date: 2016-07-15
Authors: Javier Cabrera-Perez; Stephanie A Condotta; Britnie R James; Sakeen W Kashem; Erik L Brincks; Deepa Rai; Tamara A Kucaba; Vladimir P Badovinac; Thomas S Griffith Journal: J Immunol Date: 2015-01-16 Impact factor: 5.422
Authors: K Risso; G Kumar; M Ticchioni; C Sanfiorenzo; J Dellamonica; F Guillouet-de Salvador; G Bernardin; C-H Marquette; P-M Roger Journal: Eur J Clin Microbiol Infect Dis Date: 2015-02-05 Impact factor: 3.267