| Literature DB >> 22820336 |
Adrian J Tett1, Steven J Rudder, Alexandre Bourdès, Ramakrishnan Karunakaran, Philip S Poole.
Abstract
Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.Entities:
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Year: 2012 PMID: 22820336 PMCID: PMC3457481 DOI: 10.1128/AEM.01188-12
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792