BACKGROUND: Kynurenic acid (KYNA), a tryptophan metabolite, was found in the mucus of rat small intestine. However, its role in the gastrointestinal tract is still not fully elucidated. METHODS: To verify whether KYNA affects cell cycle regulators, the protein expression of cyclin-dependent kinase inhibitor p21 Waf1/Cip1 was investigated in colon adenocarcinoma HT-29 cells exposed to KYNA. MTT, BrdU assay and siRNA technology were used to evaluate the effect of KYNA on cancer cell proliferation. RESULTS: KYNA significantly enhanced the expression of p21 Waf1/Cip1. Importantly, the overexpression of this protein was involved in inhibition of proliferation and DNA synthesis in HT-29 cells. CONCLUSIONS: KYNA may be considered as a potential chemoprevention agent against colon cancer.
BACKGROUND:Kynurenic acid (KYNA), a tryptophan metabolite, was found in the mucus of rat small intestine. However, its role in the gastrointestinal tract is still not fully elucidated. METHODS: To verify whether KYNA affects cell cycle regulators, the protein expression of cyclin-dependent kinase inhibitor p21 Waf1/Cip1 was investigated in colon adenocarcinoma HT-29 cells exposed to KYNA. MTT, BrdU assay and siRNA technology were used to evaluate the effect of KYNA on cancer cell proliferation. RESULTS:KYNA significantly enhanced the expression of p21 Waf1/Cip1. Importantly, the overexpression of this protein was involved in inhibition of proliferation and DNA synthesis in HT-29 cells. CONCLUSIONS:KYNA may be considered as a potential chemoprevention agent against colon cancer.
Authors: Amrita K Cheema; Irene Maier; Tyrone Dowdy; Yiwen Wang; Rajbir Singh; Paul M Ruegger; James Borneman; Albert J Fornace; Robert H Schiestl Journal: PLoS One Date: 2016-04-13 Impact factor: 3.240
Authors: Katarzyna Walczak; Ewa Langner; Karolina Szalast; Anna Makuch-Kocka; Piotr Pożarowski; Tomasz Plech Journal: Molecules Date: 2020-04-03 Impact factor: 4.411