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Literature DB >> 22810424

Single-cell electroporation using a multifunctional pipette.

Alar Ainla¹, Shijun Xu, Nicolas Sanchez, Gavin D M Jeffries, Aldo Jesorka.

Abstract

We present here a novel platform combination, using a multifunctional pipette to individually electroporate single-cells and to locally deliver an analyte, while in their culture environment. We demonstrate a method to fabricate low-resistance metallic electrodes into a PDMS pipette, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode.

Entities: CellLine Chemical Disease Gene

Mesh：

Substances：

Year: 2012 PMID： 22810424 PMCID： PMC3805499 DOI： 10.1039/c2lc40563f

Source DB: PubMed Journal: Lab Chip ISSN： 1473-0189 Impact factor: 6.799

27 in total

1. Selective introduction of antisense oligonucleotides into single adult CNS progenitor cells using electroporation demonstrates the requirement of STAT3 activation for CNTF-induced gliogenesis.

Authors: M A Aberg; F Ryttsén; G Hellgren; K Lindell; L E Rosengren; A J MacLennan; B Carlsson; O Orwar; P S Eriksson
Journal: Mol Cell Neurosci Date: 2001-03 Impact factor: 4.314

Review 2. Single-cell electroporation.

Authors: Jessica Olofsson; Kerstin Nolkrantz; Frida Ryttsén; Bradley A Lambie; Stephen G Weber; Owe Orwar
Journal: Curr Opin Biotechnol Date: 2003-02 Impact factor: 9.740

3. Continuous sorting and separation of microparticles by size using AC dielectrophoresis in a PDMS microfluidic device with 3-D conducting PDMS composite electrodes.

Authors: Nuttawut Lewpiriyawong; Chun Yang; Yee Cheong Lam
Journal: Electrophoresis Date: 2010-08 Impact factor: 3.535

4. A single cell electroporation chip.

Authors: Michelle Khine; Adrian Lau; Cristian Ionescu-Zanetti; Jeonggi Seo; Luke P Lee
Journal: Lab Chip Date: 2004-09-22 Impact factor: 6.799

5. Automated single-cell electroporation.

Authors: Chilman Bae; Peter J Butler
Journal: Biotechniques Date: 2006-10 Impact factor: 1.993

6. Scanning electroporation of selected areas of adherent cell cultures.

Authors: Jessica Olofsson; Mikael Levin; Anette Strömberg; Stephen G Weber; Frida Ryttsén; Owe Orwar
Journal: Anal Chem Date: 2007-05-19 Impact factor: 6.986

Review 7. Single-cell electroporation.

Authors: Manyan Wang; Owe Orwar; Jessica Olofsson; Stephen G Weber
Journal: Anal Bioanal Chem Date: 2010-05-23 Impact factor: 4.142

Single-cell electroporation using a multifunctional pipette.

1. Selective introduction of antisense oligonucleotides into single adult CNS progenitor cells using electroporation demonstrates the requirement of STAT3 activation for CNTF-induced gliogenesis.

Review 2. Single-cell electroporation.

3. Continuous sorting and separation of microparticles by size using AC dielectrophoresis in a PDMS microfluidic device with 3-D conducting PDMS composite electrodes.

4. A single cell electroporation chip.

5. Automated single-cell electroporation.

6. Scanning electroporation of selected areas of adherent cell cultures.

Review 7. Single-cell electroporation.

Review 8. Breaking the barrier: methods for reversible permeabilization of cellular membranes.

9. A multifunctional pipette.

10. Altering the biochemical state of individual cultured cells and organelles with ultramicroelectrodes.

1. Microfluidic sampling system for tissue analytics.

2. Spatial characterization of a multifunctional pipette for drug delivery in hippocampal brain slices.

3. Rapid tool for cell nanoarchitecture integrity assessment.

4. Microbial Nanocellulose Printed Circuit Boards for Medical Sensing.