Literature DB >> 2280685

The influence of the Klebsiella pneumoniae regulatory gene nifL upon the transcriptional activator protein NifA.

E Morett1, R Kreutzer, W Cannon, M Buck.   

Abstract

The influence of the Klebsiella pneumoniae nifL gene product upon the interaction of the transcriptional activator protein NifA with the nifH promoter has been examined using in vivo dimethylsulphate 'footprinting'. Binding of NifA to the upstream activator sequence (UAS) of the nifH promoter in the presence of the NifL protein was observed under nitrogen-limiting growth conditions. Growth in the presence of NH4+ or addition of NH4+ to nitrogen-limited cells diminished the interaction of NifA with the UAS when NifL was present. Repression of nif transcription by NifL may therefore involve an interaction between NifL and NifA which reduces the affinity of NifA for the UAS.

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Year:  1990        PMID： 2280685     DOI： 10.1111/j.1365-2958.1990.tb00704.x

Source DB:  PubMed          Journal:  Mol Microbiol        ISSN： 0950-382X            Impact factor:   3.501


  14 in total

1.  High-resolution physical map of the Sinorhizobium meliloti 1021 pSyma megaplasmid.

Authors:  F Barloy-Hubler; D Capela; M J Barnett; S Kalman; N A Federspiel; S R Long; F Galibert
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

2.  Purification of the regulatory protein AlgR1 and its binding in the far upstream region of the algD promoter in Pseudomonas aeruginosa.

Authors:  J Kato; A M Chakrabarty
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

3.  Identification of a positive transcription regulatory element within the coding region of the nifLA operon in Azotobacter vinelandii.

Authors:  Ranjana Mitra; Hirendra K Das; Aparna Dixit
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

4.  Transcription termination within the regulatory nifLA operon of Klebsiella pneumoniae.

Authors:  F Govantes; E Santero
Journal:  Mol Gen Genet       Date:  1996-03-07

5.  Influence of oxygen on DNA binding, positive control, and stability of the Bradyrhizobium japonicum NifA regulatory protein.

Authors:  E Morett; H M Fischer; H Hennecke
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

6.  Excretion of ammonium by a nifL mutant of Azotobacter vinelandii fixing nitrogen.

Authors:  A Bali; G Blanco; S Hill; C Kennedy
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

7.  Characterisation of mutations in the Klebsiella pneumoniae nitrogen fixation regulatory gene nifL which impair oxygen regulation.

Authors:  C Sidoti; G Harwood; R Ackerman; J Coppard; M Merrick
Journal:  Arch Microbiol       Date:  1993       Impact factor: 2.552

8.  The isolated catalytic domain of NIFA, a bacterial enhancer-binding protein, activates transcription in vitro: activation is inhibited by NIFL.

Authors:  D K Berger; F Narberhaus; S Kustu
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-04       Impact factor: 11.205

9.  Regulation of nif gene expression in Enterobacter agglomerans: nucleotide sequence of the nifLA operon and influence of temperature and ammonium on its transcription.

Authors:  D Siddavattam; H D Steibl; R Kreutzer; W Klingmüller
Journal:  Mol Gen Genet       Date:  1995-12-20

Review 10.  Nitrogen control in bacteria.

Authors:  M J Merrick; R A Edwards
Journal:  Microbiol Rev       Date:  1995-12
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