Kaigui Cai1, Ying Luo, Lixiang Li, Yuehui Liu. 1. Department of Otorhinolaryngology-Head and Neck Surgery, People's Hospital of Guangzhou Huadu District, Guangzhou 510800, China.
Abstract
OBJECTIVE: To investigate the expression of MCM2, Ki-67 and Rb and its biological characteristic in human laryngeal squamous cell carcinomas(LSCC). METHOD: The expression of MCM2 protein and Rb protein were detected in 60 cases of LSCC, 10 cases of precarcinoma, 10 cases of vocal cord polyps and 10 cases of normal laryngeal tissues, and Ki-67 protein were detected in 60 cases of LSCC and 10 cases of normal laryngeal tissues by Elivision plus immunohistochemical staining, and analyze their relations with clinicopathological characteristics. RESULT: The positive expression rate of MCM2 in LSCC was significantly higher than that in precarcinoma and normal laryngeal tissues (P < 0.05), and was positively correlated with pathological grades, clinical stages and lymph node metastases (P < 0.05) of LSCC. The positive expression rate of Rb protein in LSCC was significantly lower than that in precarcinoma and normal laryngeal tissues (P < 0.05). The expression level of MCM2 in LSCC was negatively corelated with Rb (r = -0.542, P < 0.05), the expression level of Ki-67 in LSCC (76.67%) was significantly higher than that in normal laryngeal tissues (30.00%) (P < 0.01) and the expression level of MCM2 in LSCC was positively corelated with Ki-67(r = 0.596, P < 0.01). The LI of MCM2 in the 3-year survival rate of LSCC was significantly lower than that in Ki-67 (P < 0.05). CONCLUSION: Over expression of MCM2 and loss of Rb protein were related to the carcinogenesis and development of LSCC. The determination of MCM2 can be an index for estimating the level of malignancy and prognosis of LSCC.
OBJECTIVE: To investigate the expression of MCM2, Ki-67 and Rb and its biological characteristic in humanlaryngeal squamous cell carcinomas(LSCC). METHOD: The expression of MCM2 protein and Rb protein were detected in 60 cases of LSCC, 10 cases of precarcinoma, 10 cases of vocal cord polyps and 10 cases of normal laryngeal tissues, and Ki-67 protein were detected in 60 cases of LSCC and 10 cases of normal laryngeal tissues by Elivision plus immunohistochemical staining, and analyze their relations with clinicopathological characteristics. RESULT: The positive expression rate of MCM2 in LSCC was significantly higher than that in precarcinoma and normal laryngeal tissues (P < 0.05), and was positively correlated with pathological grades, clinical stages and lymph node metastases (P < 0.05) of LSCC. The positive expression rate of Rb protein in LSCC was significantly lower than that in precarcinoma and normal laryngeal tissues (P < 0.05). The expression level of MCM2 in LSCC was negatively corelated with Rb (r = -0.542, P < 0.05), the expression level of Ki-67 in LSCC (76.67%) was significantly higher than that in normal laryngeal tissues (30.00%) (P < 0.01) and the expression level of MCM2 in LSCC was positively corelated with Ki-67(r = 0.596, P < 0.01). The LI of MCM2 in the 3-year survival rate of LSCC was significantly lower than that in Ki-67 (P < 0.05). CONCLUSION: Over expression of MCM2 and loss of Rb protein were related to the carcinogenesis and development of LSCC. The determination of MCM2 can be an index for estimating the level of malignancy and prognosis of LSCC.
Authors: Shalaka Joshi; Johnathan Watkins; Patrycja Gazinska; John P Brown; Cheryl E Gillett; Anita Grigoriadis; Sarah E Pinder Journal: BMC Cancer Date: 2015-07-25 Impact factor: 4.430