Li Zhang1, Li-Pan Peng, Nan Wu, Le-Ping Li. 1. Department of Gastrointestinal Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China. pzzl@sina.com
Abstract
OBJECTIVE: To review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC). DATA SOURCES: The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture". STUDY SELECTION: Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D. RESULTS: Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems. CONCLUSIONS: Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.
OBJECTIVE: To review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC). DATA SOURCES: The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture". STUDY SELECTION: Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D. RESULTS: Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems. CONCLUSIONS: Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.
Authors: Carolina Assis P Vilela; Lucas Eduardo B Souza; Rubens C Siqueira; Rodrigo T Calado; Dimas T Covas; Jayter S Paula Journal: Stem Cell Res Ther Date: 2018-06-13 Impact factor: 6.832