Fang Yang1, Bo Xu, Junjun Li, Zunxi Huang. 1. School of Life Sciences, Yunnan Normal University, Kunming 650500, China. fangyang09@163.com
Abstract
OBJECTIVE: To study whether Termitomyces albuminosus can degrade lignocelluloses and to understand the symbiotic relationship between termite mushroom and fungus-growing termite. METHODS: cDNA library of T. albuminosus was sequenced by the Roche 454 GS FLX Titanium platform, and the diverse enzymes relevant to degradation of cellulose and lignin of symbiotic fungus T. albuminosus were analyzed. RESULTS: Eighth sequencing run resulted in a total of 82386 reads (express sequence tags, EST). After removing the vector and primer sequences, the remained 54410 reads were assembled into 3301 contigs and 3193 singletons. Comparing sequence similarity with known proteins, these sequences, representing approximately 2681 unique genes, were successfully annotated using BLAST searches (E-value < or = 1e(-10)) against the Nr, SwissProt and CDD databases. The T. albuminosus transcripts included 33 enzymes putatively involved in cellulose and hemicelluloses biodegradation. 5 enzymes could hydrolyze cellulose and others had catalytic activities for degradation of hemicelluloses, starch and glycogen and chitin. Moreover, four genes encoding laccases and a single aryl-alcohol oxidase which could degrade lignin were also identified. These results revealed symbiosis fungus T. albuminosus had many laccases and possibly decomposed phenolic compounds from plant litter. CONCLUSIONS: Data presented in this study indicated that T. albuminosus had the ability to degrade lignin, which made cellulose more easily degraded by the cellulase produced by fungus-growing termite.
OBJECTIVE: To study whether Termitomyces albuminosus can degrade lignocelluloses and to understand the symbiotic relationship between termite mushroom and fungus-growing termite. METHODS: cDNA library of T. albuminosus was sequenced by the Roche 454 GS FLX Titanium platform, and the diverse enzymes relevant to degradation of cellulose and lignin of symbiotic fungus T. albuminosus were analyzed. RESULTS: Eighth sequencing run resulted in a total of 82386 reads (express sequence tags, EST). After removing the vector and primer sequences, the remained 54410 reads were assembled into 3301 contigs and 3193 singletons. Comparing sequence similarity with known proteins, these sequences, representing approximately 2681 unique genes, were successfully annotated using BLAST searches (E-value < or = 1e(-10)) against the Nr, SwissProt and CDD databases. The T. albuminosus transcripts included 33 enzymes putatively involved in cellulose and hemicelluloses biodegradation. 5 enzymes could hydrolyze cellulose and others had catalytic activities for degradation of hemicelluloses, starch and glycogen and chitin. Moreover, four genes encoding laccases and a single aryl-alcohol oxidase which could degrade lignin were also identified. These results revealed symbiosis fungus T. albuminosus had many laccases and possibly decomposed phenolic compounds from plant litter. CONCLUSIONS: Data presented in this study indicated that T. albuminosus had the ability to degrade lignin, which made cellulose more easily degraded by the cellulase produced by fungus-growing termite.
Authors: Michael Poulsen; Haofu Hu; Cai Li; Zhensheng Chen; Luohao Xu; Saria Otani; Sanne Nygaard; Tania Nobre; Sylvia Klaubauf; Philipp M Schindler; Frank Hauser; Hailin Pan; Zhikai Yang; Anton S M Sonnenberg; Z Wilhelm de Beer; Yong Zhang; Michael J Wingfield; Cornelis J P Grimmelikhuijzen; Ronald P de Vries; Judith Korb; Duur K Aanen; Jun Wang; Jacobus J Boomsma; Guojie Zhang Journal: Proc Natl Acad Sci U S A Date: 2014-09-22 Impact factor: 11.205
Authors: Norasfaliza Rahmad; Jameel R Al-Obaidi; Noraswati Mohd Nor Rashid; Ng Boon Zean; Mohd Hafis Yuswan Mohd Yusoff; Nur Syahidah Shaharuddin; Nor Azreen Mohd Jamil; Norihan Mohd Saleh Journal: Biol Res Date: 2014-07-03 Impact factor: 5.612