| Literature DB >> 22798652 |
William J Wolfgang1,2, Teresa V Passaretti2, Reashma Jose2, Jocelyn Cole2, An Coorevits3,4, Andrea N Carpenter2, Sherly Jose2, Anita Van Landschoot3,4, Jacques Izard5,6, Donna J Kohlerschmidt2, Peter Vandamme3, Floyd E Dewhirst5,6, Mark A Fisher7,8, Kimberlee A Musser1,2.
Abstract
A polyphasic analysis was undertaken of seven independent isolates of gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7-100 %, and the closest species with a validly published name was Neisseria lactamica (96.9 % similarity to the type strain). DNA-DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbour, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the novel species belongs in the genus Neisseria. The predominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C18 : 1ω7c. The cellular fatty acid profile, together with other phenotypic characters, further supports the inclusion of the novel species in the genus Neisseria. The name Neisseria oralis sp. nov. (type strain 6332(T) = DSM 25276(T) = LMG 26725(T)) is proposed.Entities:
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Year: 2012 PMID： 22798652 PMCID： PMC3709538 DOI： 10.1099/ijs.0.041731-0
Source DB: PubMed Journal: Int J Syst Evol Microbiol ISSN： 1466-5026 Impact factor: 2.747