OBJECTIVE: To analyze the expression of peroxisome proliferator-activated receptor-γ1 and 2 (PPARγ1 and 2), 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), and leptin in adipose tissue (AT) of obese women during weight loss following Roux-en-Y gastric bypass (RYGB) and to compare these levels with those obtained in AT of nonobese subjects. METHODS: Gene expression was determined by real-time RT-PCR prior to surgery and at 3, 6, and 12 months after RYGB. RESULTS: All obese patients lost weight, reaching a mean BMI of 29.3 ± 1.0 kg/m(2) at 1 year after surgery (-33.9 ± 1.5% of their initial body weight). In obese subjects leptin and 11βHSD1 were over-expressed, whereas PPARγ1 was expressed at lower levels compared to controls. After surgery, leptin and 11βHSD1 gene expression decreased, whereas PPARγ1 expression increased. At 12 months after RYGB, these 3 genes had reached levels similar to the controls. In contrast, PPARγ2 gene expression was not different between groups and types of tissue and remained unchanged during weight loss. We found a positive correlation between BMI and levels of gene expression of leptin and 11βHSD1. CONCLUSION: Gene expression of leptin, PPARγ1, and 11βHSD1 in AT is modified in human obesity. This default is completely corrected by RYGB.
OBJECTIVE: To analyze the expression of peroxisome proliferator-activated receptor-γ1 and 2 (PPARγ1 and 2), 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1), and leptin in adipose tissue (AT) of obesewomen during weight loss following Roux-en-Y gastric bypass (RYGB) and to compare these levels with those obtained in AT of nonobese subjects. METHODS: Gene expression was determined by real-time RT-PCR prior to surgery and at 3, 6, and 12 months after RYGB. RESULTS: All obesepatients lost weight, reaching a mean BMI of 29.3 ± 1.0 kg/m(2) at 1 year after surgery (-33.9 ± 1.5% of their initial body weight). In obese subjects leptin and 11βHSD1 were over-expressed, whereas PPARγ1 was expressed at lower levels compared to controls. After surgery, leptin and 11βHSD1 gene expression decreased, whereas PPARγ1 expression increased. At 12 months after RYGB, these 3 genes had reached levels similar to the controls. In contrast, PPARγ2 gene expression was not different between groups and types of tissue and remained unchanged during weight loss. We found a positive correlation between BMI and levels of gene expression of leptin and 11βHSD1. CONCLUSION: Gene expression of leptin, PPARγ1, and 11βHSD1 in AT is modified in humanobesity. This default is completely corrected by RYGB.
Authors: Alexander Jürets; Bianca Karla Itariu; Magdalena Keindl; Gerhard Prager; Felix Langer; Viktor Grablowitz; Maximilian Zeyda; Thomas Michael Stulnig Journal: Obes Surg Date: 2017-06 Impact factor: 4.129
Authors: Samar H K Tareen; Michiel E Adriaens; Ilja C W Arts; Theo M de Kok; Roel G Vink; Nadia J T Roumans; Marleen A van Baak; Edwin C M Mariman; Chris T Evelo; Martina Kutmon Journal: Genes (Basel) Date: 2018-10-29 Impact factor: 4.096