Literature DB >> 2279146

Separation of glycoprotein-N-glycans by high-pH anion-exchange chromatography.

G Pfeiffer1, H Geyer, R Geyer, I Kalsner, P Wendorf.   

Abstract

A series of high-mannose and complex type glycoprotein-N-glycans was subjected to high-pH anion-exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side-chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species.

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Year:  1990        PMID： 2279146     DOI： 10.1002/bmc.1130040505

Source DB:  PubMed          Journal:  Biomed Chromatogr        ISSN： 0269-3879            Impact factor:   1.902


  5 in total

1.  Structural elucidation of zwitterionic carbohydrates derived from glycosphingolipids of the porcine parasitic nematode Ascaris suum.

Authors:  Claudia H Friedl; Günter Lochnit; Ulrich Zähringer; Ute Bahr; Rudolf Geyer
Journal:  Biochem J       Date:  2003-01-01       Impact factor: 3.857

2.  Glycosylation of the thrombin-like serine protease ancrod from Agkistrodon rhodostoma venom. Oligosaccharide substitution pattern at each N-glycosylation site.

Authors:  G Pfeiffer; D Linder; K H Strube; R Geyer
Journal:  Glycoconj J       Date:  1993-06       Impact factor: 2.916

3.  Comparison of the carbohydrate moieties of recombinant soluble Fc epsilon receptor (sFc epsilon RII/sCD23) expressed in Saccharomyces cerevisiae and Chinese hamster ovary cells. Different O-glycosylation sites are used by yeast and mammalian cells.

Authors:  I Kalsner; F J Schneider; R Geyer; H Ahorn; I Maurer-Fogy
Journal:  Glycoconj J       Date:  1992-08       Impact factor: 2.916

4.  Carbohydrate structure of human pancreatic elastase 1.

Authors:  P Wendorf; D Linder; A Sziegoleit; R Geyer
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

5.  Selective reentry of recycling cell surface glycoproteins to the biosynthetic pathway in human hepatocarcinoma HepG2 cells.

Authors:  B Volz; G Orberger; S Porwoll; H P Hauri; R Tauber
Journal:  J Cell Biol       Date:  1995-08       Impact factor: 10.539
  5 in total

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