Use of fluorescent in situ hybridisation to confirm trisomy of chromosome region 1q32-qter as the sole karyotypic defect in a colon cancer cell line.

The sole chromosome defect in a colon cancer cell line derived from a patient with inherited nonpolyposis colorectal cancer was karyotypically designated as 46,XY,-13,+der(13)t(1;13)(q32.1;p11) on the basis of banding homology. We have obtained molecular confirmation that the additional chromosome material is derived from chromosome region 1q32-qter by the use of a highly specific fluorescent in situ hybridisation technique on G-banded chromosomes and also by Southern hybridisation.