| Literature DB >> 22785193 |
M Doumith1, M J Day, R Hope, J Wain, N Woodford.
Abstract
Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.Entities:
Mesh:
Year: 2012 PMID: 22785193 PMCID: PMC3421818 DOI: 10.1128/JCM.01468-12
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948