BACKGROUND: The immune system changes with age. In this study we characterized immune changes by performing immunologic screening profiles on ageing individuals. METHODS: This study was performed at Akdeniz University, in the Faculty of Medicine, Department of Immunology. Healthy volunteers consisted of a younger group (22 donors) and an older group (45 individuals). All subjects had no serious health problems (i.e. chronic heart, lung, liver or immunological diseases) and were taking no prescribed medications. Flow cytometry analysis was used to evaluate CD3, CD4, CD8, CD16, CD19, CD28, CD40, CD45, CD56, CD80, CD86, CTLA-4 and ELISA for IL-1 beta, IL-2, IL-6, IL-10, IFN-gamma, TNF-alpha expression In addition, NK activity and induced cytokine expression (by bioassay and ELISA, respectively) were evaluated. RESULTS: No statistical differences were observed between the two groups in expression of CD3, CD8, CD19, CD80, CD86, CD16, CD 56, or CD28. A higher frequency of expression of CD4, CTLA-4, CD40, and CD45 was seen in older subjects by comparison with younger subjects. Cytokine profiles expressed by stimulated monocytes and lymphocytes from the two groups showed no difference in IL-1 beta, IL-2, IL-6, IL-10, TNF-alpha, and IFN-gamma production levels. CONCLUSIONS: We found increased expression levels of CD40 and CD45 levels in healthy older (age: 59.42 +/- 5.89) versus younger individuals (age: 30.32 +/- 2.29). CTLA-4 expression levels were also higher in older subjects, with no difference in CD28 expression levels between younger/older individuals.
BACKGROUND: The immune system changes with age. In this study we characterized immune changes by performing immunologic screening profiles on ageing individuals. METHODS: This study was performed at Akdeniz University, in the Faculty of Medicine, Department of Immunology. Healthy volunteers consisted of a younger group (22 donors) and an older group (45 individuals). All subjects had no serious health problems (i.e. chronic heart, lung, liver or immunological diseases) and were taking no prescribed medications. Flow cytometry analysis was used to evaluate CD3, CD4, CD8, CD16, CD19, CD28, CD40, CD45, CD56, CD80, CD86, CTLA-4 and ELISA for IL-1 beta, IL-2, IL-6, IL-10, IFN-gamma, TNF-alpha expression In addition, NK activity and induced cytokine expression (by bioassay and ELISA, respectively) were evaluated. RESULTS: No statistical differences were observed between the two groups in expression of CD3, CD8, CD19, CD80, CD86, CD16, CD 56, or CD28. A higher frequency of expression of CD4, CTLA-4, CD40, and CD45 was seen in older subjects by comparison with younger subjects. Cytokine profiles expressed by stimulated monocytes and lymphocytes from the two groups showed no difference in IL-1 beta, IL-2, IL-6, IL-10, TNF-alpha, and IFN-gamma production levels. CONCLUSIONS: We found increased expression levels of CD40 and CD45 levels in healthy older (age: 59.42 +/- 5.89) versus younger individuals (age: 30.32 +/- 2.29). CTLA-4 expression levels were also higher in older subjects, with no difference in CD28 expression levels between younger/older individuals.
Authors: Bernhard Schermer; Valerie Bartels; Peter Frommolt; Bianca Habermann; Fabian Braun; Joachim L Schultze; Marianne Roodbergen; Jan Hj Hoeijmakers; Björn Schumacher; Peter Nürnberg; Martijn Et Dollé; Thomas Benzing; Roman-Ulrich Müller; Christine E Kurschat Journal: BMC Genomics Date: 2013-08-16 Impact factor: 3.969
Authors: Vanna Chiarion Sileni; Jacopo Pigozzo; Paolo Antonio Ascierto; Antonio Maria Grimaldi; Michele Maio; Lorenza Di Guardo; Paolo Marchetti; Francesco de Rosa; Carmen Nuzzo; Alessandro Testori; Emilia Cocorocchio; Maria Grazia Bernengo; Michele Guida; Riccardo Marconcini; Barbara Merelli; Giorgio Parmiani; Gaetana Rinaldi; Massimo Aglietta; Marco Grosso; Paola Queirolo Journal: J Exp Clin Cancer Res Date: 2014-04-04