| Literature DB >> 22771536 |
Olaf Hardt1, Stefan Wild, Ilka Oerlecke, Kay Hofmann, Shujun Luo, Yvonne Wiencek, Eva Kantelhardt, Christoph Vess, Geoffrey P Smith, Gary P Schroth, Andreas Bosio, Jürgen Dittmer.
Abstract
We performed next generation sequencing- and microarray-based gene expression profiling of CD44(+)/CD24(-)/CD45(-) breast CSCs (cancer stem cells) isolated from primary ERα-positive breast cancer. By combining semi-automated dissociation of human tumor tissue, magnetic cell sorting and cDNA amplification less than 500 CSCs were required for transcriptome analyses. Besides overexpressing genes involved in maintenance of stemness, the CSCs showed higher levels of genes that drive the PI3K pathway, including EGFR, HB-EGF, PDGFRA/B, PDGF, MET, PIK3CA, PIK3R1 and PIK3R2. This suggests that, in CSCs of ERα-positive breast cancer, the PI3K pathway which is involved in endocrine resistance is hyperactive.Entities:
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Year: 2012 PMID： 22771536 DOI： 10.1016/j.canlet.2012.06.010
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679