Literature DB >> 2277118

Efficient large-scale purification of non-histone chromosomal proteins HMG1 and HMG2 by using Polybuffer-exchanger PBE94.

Y Adachi1, S Mizuno, M Yoshida.   

Abstract

A method for the efficient and practical large-scale purification of high-mobility group (HMG) non-histone chromosomal proteins, HMG1 and HMG2, from porcine thymus applying Polybuffer-exchanger PBE94 gel as anion-exchanger has been developed. This method affords higher resolution, purity and yield, than the conventional procedure of CM-Sephadex C-25 ion-exchange column chromatography. Furthermore, use of Polybuffer-exchanger PBE94 column chromatography led to direct preparation of HMG1 and HMG2 from loosely bound non-histone chromosomal protein fraction of chromatin without prefractional precipitation with trichloroacetic acid or prior extraction with perchloric acid. Thus, the application of PBE94 gel as an anion-exchanger to the subfractionation of other kinds of homologous protein is possible.

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Year:  1990        PMID： 2277118     DOI： 10.1016/s0378-4347(00)82300-2

Source DB:  PubMed          Journal:  J Chromatogr


  12 in total

1.  Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases.

Authors:  Deepa Subramanian; Jack D Griffith
Journal:  Nucleic Acids Res       Date:  2002-06-01       Impact factor: 16.971

2.  In vitro processing of the 3'-overhanging DNA in the postcleavage complex involved in V(D)J joining.

Authors:  Tadashi Nishihara; Fumikiyo Nagawa; Hirofumi Nishizumi; Masami Kodama; Satoshi Hirose; Reiko Hayashi; Hitoshi Sakano
Journal:  Mol Cell Biol       Date:  2004-05       Impact factor: 4.272

3.  Footprint analysis of recombination signal sequences in the 12/23 synaptic complex of V(D)J recombination.

Authors:  Fumikiyo Nagawa; Masami Kodama; Tadashi Nishihara; Kei-Ichiro Ishiguro; Hitoshi Sakano
Journal:  Mol Cell Biol       Date:  2002-10       Impact factor: 4.272

4.  Competition between HMG-I(Y), HMG-1 and histone H1 on four-way junction DNA.

Authors:  D A Hill; R Reeves
Journal:  Nucleic Acids Res       Date:  1997-09-01       Impact factor: 16.971

5.  Native versus recombinant high-mobility group B1 proteins: functional activity in vitro.

Authors:  Klaus Zimmermann; Dirk Völkel; Sabrina Pable; Thomas Lindner; Franziska Kramberger; Soheyl Bahrami; Friedrich Scheiflinger
Journal:  Inflammation       Date:  2004-08       Impact factor: 4.092

6.  A novel activity of HMG domains: promotion of the triple-stranded complex formation between DNA containing (GGA/TCC)11 and d(GGA)11 oligonucleotides.

Authors:  T Suda; Y Mishima; K Takayanagi; H Asakura; S Odani; R Kominami
Journal:  Nucleic Acids Res       Date:  1996-12-01       Impact factor: 16.971

7.  The major chromatin protein histone H1 binds preferentially to cis-platinum-damaged DNA.

Authors:  J Yaneva; S H Leuba; K van Holde; J Zlatanova
Journal:  Proc Natl Acad Sci U S A       Date:  1997-12-09       Impact factor: 11.205

8.  Determinants of HMGB proteins required to promote RAG1/2-recombination signal sequence complex assembly and catalysis during V(D)J recombination.

Authors:  Yan Dai; Ben Wong; Yi-Meng Yen; Marjorie A Oettinger; Jongbum Kwon; Reid C Johnson
Journal:  Mol Cell Biol       Date:  2005-06       Impact factor: 4.272

9.  The DNA-bending protein HMG-1 enhances progesterone receptor binding to its target DNA sequences.

Authors:  S A Oñate; P Prendergast; J P Wagner; M Nissen; R Reeves; D E Pettijohn; D P Edwards
Journal:  Mol Cell Biol       Date:  1994-05       Impact factor: 4.272

10.  Evidence for a shared structural role for HMG1 and linker histones B4 and H1 in organizing chromatin.

Authors:  K Nightingale; S Dimitrov; R Reeves; A P Wolffe
Journal:  EMBO J       Date:  1996-02-01       Impact factor: 11.598
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